计算溶液所需的质量、体积或浓度。
草酰乙酸脱羧酶 (OAD)来源于假单胞菌属
有货
为什么选择此级别
EnzymoPure™,重组 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Oxaloacetate decarboxylase is a family of metal-dependent lyases that catalyze the irreversible decarboxylation of oxaloacetate to pyruvate and carbon dioxide. It is widely distributed in bacteria, archaea, and some eukaryotes. The enzyme participates in the tricarboxylic acid cycle anaplerotic reactions, energy metabolism, and the synthesis of small molecular carbon skeletons. Some membrane-bound isoforms can couple with sodium ion transmembrane transport to form an ion gradient for energy supply. Based on structure and localization, it can be divided into cytoplasmic soluble form (EC 4.1.1.112) and membrane-bound Na⁺-dependent form (EC 7.2.4.2). The former mostly relies on Mn²⁺/Mg²⁺, while the latter contains a biotin cofactor and functions as a sodium ion pump. Applications This enzyme is useful for enzymatic determination of AST when coupled with pyruvate oxidase. Preparation and Specification Appearance: White to off white amorphous powder, lyophilized Specific activity: More than 2300 U/mg solid Contaminants: AST (GOT)Less than 0.005 % (U/U) CatalaseLess than 0.3 % (U/U) Properties Molecular weight: 31 kDa (SDS–PAGE) , 120 kDa (Superdex 200) Isoelectric point: pH 5.16 Michaelis constants: Oxaloacetate 3.3 × 10^-3M Optimum pH: 7.5–8.5 pH stability: 7.5–9.0 (50℃, 3 hr.) Optimum temperature: 40–50℃ Thermal stability: Stable at 50℃ and below (pH 7.0, 10 min) Storage stability: At least one year at －20℃Activators: Mg2+, Mn2+ Inhibitors: Sodium dodecylsulfate, Sodium laurylbenzen sulfonate
规格
产品名称
草酰乙酸脱羧酶 (OAD)来源于假单胞菌属
规格或纯度
EnzymoPure™, 重组, ≥2300 U/mg enzyme powder
CAS编号和信息
9024-98-0
酶学委员会编号
EC 4.1.1.3
分子类型
酶
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
浓度
≥2300 U/mg enzyme powder
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
单位定义
One unit is defined as the amount of enzyme which produces 1 μmole of pyruvate per minute at 25℃ under the conditions specified in the assay procedure.
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
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