计算溶液所需的质量、体积或浓度。
油红O异丙醇饱和液
有货
为什么选择此级别
BioReagent,生物染色剂 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
中性脂肪染色经常采用苏丹Ⅱ、苏丹Ⅲ、苏丹Ⅳ、苏丹黑 B、油红O法等。传统方法采用苏丹染料，最近发现偶氮染料油红O更适合脂肪的染色。油红O是很强的脂溶剂和染脂剂，较易与甘油三脂结合呈小脂滴状，与磷脂结合力稍差。其染色原理一般认为是物理上的溶液作用或吸附作用，借溶液作用使脂肪染色。油红O染色主要用于显示组织器官的脂肪变性和类脂质的异常沉着，常发生于肝、肾、心等实质脏器的脂肪变性，细胞内出现多数中性脂肪滴；鉴别和诊断脂肪组织中所发生的肿瘤及其性质。
操作步骤(仅供参考)：
1、按油红O异丙醇饱和溶液:蒸馏水=3:2 比例配制油红O染色工作液。易产生沉淀，影响观察，可按需配制后采用静置 20~40min 或 3000rpm 离心10 分钟，取上清备用。
2、冰冻切片厚度 6～10μm，不固定或 10% 福尔马林固定 10min 后水洗。
3、切片入蒸馏水中稍冲洗。
4、切片入 60%异丙醇浸洗20-30s。
5、切片入油红O染色工作液(加盖)密闭染色10-15min。
6、分色: 入 60%异丙醇内稍洗以便去除染液。
7、入蒸馏水中稍微清洗。
8、入 Mayer 苏木素染色液复染核2-5min。
9、自来水漂洗10min至核为蓝色。
10、入蒸馏水稍微清洗。
11、用滤纸吸干周围水分。
12、水溶性封固剂(甘油明胶或阿拉伯糖胶)封固，镜检。
染色结果:
中性脂肪：橙红色或橘红色
细胞核：蓝色
注意事项:
1、油红O染色工作液不够稳定，易产生沉淀，不宜提前配制。
2、如果60%异丙醇不易获得，亦可采用 70%乙醇。
3、由于脂肪易溶于有机溶剂，显示脂肪一般不能像石蜡切片一样处理，而通过冰冻切片染色来显示。
4、作为脂肪染色的冰冻切片不可太薄，过薄的切片常会使脂质丢失。
5、Mayer 苏木素染色液复染时间不能过长。
6、染色结果不能长期保存，应尽快观察及照相。
7、甘油明胶封固的样本，保存时间不长。如需长期保存需在盖玻片与载玻片交界的边缘用中性树胶封闭。
8、试剂开封后请尽快使用，以防影响后续实验效果。
9、本产品仅供科研实验，严禁他用。
Neutral fat staining frequently uses Sudan II, Sudan III, Sudan IV, Sudan Black B, Oil Red O and other methods. Traditional protocols adopt Sudan dyes, while the azo dye Oil Red O has recently been found more suitable for fat staining. Oil Red O is a potent lipid solvent and lipid stain. It readily binds to triglycerides to stain small lipid droplets, and shows relatively weaker binding affinity to phospholipids. Its staining principle is generally considered a physical dissolution or adsorption effect, whereby lipids are stained via solvent action. Oil Red O staining is mainly used to demonstrate fatty degeneration and abnormal deposition of lipoids in tissues and organs. Fatty degeneration often occurs in parenchymal organs such as liver, kidney and heart, where numerous neutral lipid droplets appear within cells. It can also be used to identify and diagnose tumors arising in adipose tissue and determine their properties.
Procedures (For reference only):
1. Prepare Oil Red O working staining solution by mixing Oil Red O isopropanol saturated solution and distilled water at a ratio of 3:2. Precipitates tend to form and interfere with observation. After preparation, let the mixture stand for 20–40 min or centrifuge at 3000 rpm for 10 min, and use the supernatant.
2. Cut frozen sections at 6–10 μm thickness. Sections may be unfixed or fixed in 10% formalin for 10 min followed by water washing.
3. Rinse sections briefly in distilled water.
4. Immerse sections in 60% isopropanol for 20–30 s.
5. Place sections in Oil Red O working solution, cover the container, and stain in a sealed environment for 10–15 min.
6. Differentiation: briefly rinse in 60% isopropanol to remove excess stain.
7. Rinse briefly in distilled water.
8. Counterstain nuclei with Mayer’s hematoxylin staining solution for 2–5 min.
9. Rinse in tap water for 10 min until nuclei turn blue.
10. Briefly wash with distilled water.
11. Blot peripheral water with filter paper.
12. Mount with water-soluble mounting medium (glycerin gelatin or arabic gum) and examine under microscope.
Staining Results:
Neutral fat: orange-red or tangerine-red
Cell nucleus: blue
Precautions:
1. Oil Red O working solution is unstable and prone to precipitation. Do not prepare it in advance.
2. If 60% isopropanol is unavailable, 70% ethanol may be used alternatively.
3. Since lipids are soluble in organic solvents, paraffin sectioning is generally not applicable for lipid demonstration; frozen section staining is adopted instead.
4. Frozen sections for lipid staining should not be too thin. Overly thin sections often cause lipid loss.
5. Counterstaining time with Mayer’s hematoxylin should not be excessive.
6. Stained samples cannot be preserved for a long time; observation and photographing should be performed promptly.
7. Specimens mounted with glycerin gelatin have a short shelf life. For long-term storage, seal the edges between coverslip and slide with neutral balsam.
8. Use reagents promptly after opening to avoid compromised experimental performance.
9. This product is for research use only. Not for other applications.
规格
规格或纯度
BioReagent, 生物染色剂
稳定性与储存
Store at room temperature long term (24 months).
英文名称
Oil Red O Isopropanol Saturated Solution
储存条件
室温
运输条件
常规运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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