计算溶液所需的质量、体积或浓度。
O-Glycosidase
有货
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Product Description
Recommended Reagents
included with:
1 vial: 5x Reaction buffer
250 mM sodium phosphate, pH 5.0
Specific Activity
One unit of O-Glycosidase is defined as the amount of enzyme required to produce 1 µmole of p-nitrophenol (pNP) in 1 minute at 37°C, pH 5.0 from p-nitrophenyl2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)- alpha-D-galactopyranoside.
Formulation
The enzyme is provided as a sterile-filtered solution in 50 mM sodium phosphate (pH 7.5).
Molecular Weight
~180,000 daltons
pH Optimum
5, active over the range 5-7.
Specifictity
Cleaves only unsubstituted Gal-ß(1-3)GalNAc-alpha disaccharides attached to the serine or threonine residues of glycoproteins or glycopeptides. Substitutions such as sialic acid, galactose, fucose or N-acetylglucosamine must first be removed with the appropriate exoglycosidase prior to treatment with O-Glycosidase.
At minimum, a neuraminidase such as Neuraminidase Au (Alpha-2-3,6,8,9), part number E-S001, is almost always required to remove sialic acids.
Purity
O-Glycosidase is tested for contaminating protease as follows: 10 µg of denatured BSA is incubated at 37°C for 24 hours with 2 µl of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation. The production host strain has been extensively tested and does not produce any detectable glycosidases.
Directions for use
1. Add up to 100 µg of glycoprotein to tube.
2. Add de-ionized water to a total of 13 µl.
3. Add 4 µl 5x Reaction Buffer 5.0.
4. Add 1 µl Neuraminidase AU (E-S001)
5. Add 2 µl O-Glycosidase.
6. Incubate at 37°C for 1 hour.
Cleavage may be monitored by SDS-PAGE if the size differential between native and de-O-glycosylated protein is sufficient for detection.
规格
别名
O-糖苷酶
英文别名
Endo-alpha-N-Acetylgalactosaminidase
规格或纯度
Specific Activity ≥ 12 U/mg;Activity ≥ 1.25 U/ml
英文名称
O-Glycosidase
来源
recombinant Streptococcus pneumoniae in E.Coli
储存条件
2-8°C储存
运输条件
低温运输
产品线
生物产品
名称和识别符
酶学委员会编号
3.2.1.97
分子类型
小分子
分子量
~180000
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
技术文档和文章
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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