PKM2-IN-8 (Compound 9b) is a pyruvate kinase M2 ( PKM2 ) inhibitor ( IC 50 = 0.31 μM). PKM2-IN-8 has potent antiproliferative activity against U87MG glioma cells. PKM2-IN-8 can induce early apoptosis and reduce lactate levels. PKM2-IN-8 can be used for the research of glioblastoma.