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蛋白质和酶
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果胶酸裂解酶

果胶酸裂解酶

Name: 果胶酸裂解酶
SKU: P1420316
Availability: InStock

CAS: 9015-75-2 货号: P1420316 EC号: 232-766-9

COA

有货

级别和纯度: 生物活性 ? ActiBioPure™ ? 天然 ? 高性能 ? EnzymoPure™ ? 来源于曲霉属; ~1400 U/mL; ≥180 U/mg protein

Accession #

Q9C2Z0

生物活性

≥180 U/mg protein

规格

库存

价格

数量

250U

P1420316-250U

现货

¥699.90

1KU

P1420316-1KU

现货

¥1,599.90

7KU

P1420316-7KU

期货

¥7,299.90

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概述规格储存与运输技术文档高级数据质检证书（CoA,COO,BSE/TSE 和分析图谱) 溶液计算器

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为什么选择此级别

ActiBioPure™,EnzymoPure™,天然,生物活性,高性能级，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

High purity Pectate lyase (Aspergillus sp.) for use in research, biochemical enzyme assays and analytical testing applications.

Suitable for pectin identification according to USP method [Pectin 9000-69-5]; Pectin Identification Procedure. Applications in carbohydrate and biofuels research.

PROPERTIES

1. ELECTROPHORETIC PURITY:

- Single major band on SDS-gel electrophoresis (MW = ~ 45,000)

2. SPECIFICITY:

Eliminative cleavage of (1,4)-α-D-galacturonan to give oligosaccharides with 4-deoxy-α-D-galact-4-enuronosyl groups at their non-reducing ends.

3. PHYSICOCHEMICAL PROPERTIES:

pH Optima: 8.0

pH Stability: 6.5-11.0

Temperature Optima: 55°C

Temperature Stability: < 55°C

4. STORAGE CONDITIONS:

The enzyme is supplied as a solution containing 50% glycerol and 0.02% (w/v) sodium azide and should

be stored below -10°C. For assay, this enzyme should be diluted in Tris.HCl buffer (100 mM), pH 8.0.

Swirl to mix the enzyme immediately prior to use.

The enzyme is used for the identification of Pectin in foodstuffs, feed and fruit juices.

规格

产品名称

果胶酸裂解酶

英文别名

规格或纯度

生物活性,ActiBioPure™,天然,高性能,EnzymoPure™,来源于曲霉属; ~1400 U/mL; ≥180 U/mg protein

生化机理

Pectinolytic enzyme consist of four classes of enzymes: pectin lyase, polygalacturonase, pectin methylesterase and rhamnogalacturonase. Among pectinolytic enzymes, pectin lyase is the most important in depolymerization of pectin, since it cleaves internal glycosidic bonds of highly methylated pectins. Favors pectate, the anion, over pectin, the methyl ester.

生物活性

≥180 U/mg protein

种属

Aspergillus sp.

来源

天然

CAS编号和信息

9015-75-2

酶学委员会编号

EC 4.2.2.2

分子类型

酶

产品线

生物产品

储存与运输

物理形态

液体

储存缓冲液

50% Glycerol, 0.02% (w/v) Sodium azide.

浓度

来源于曲霉属; ~1400 U/mL; ≥180 U/mg protein

储存条件

-20°C储存,避免反复冻融

运输条件

超低温运输

稳定性与储存

长期储存-20℃（24个月）；收货后建议分装，避免反复冻融。

单位定义

One Unit of pectate lyase activity is defined as the amount of enzyme required to release one μmole of galacturonic acid per minute from polygalacturonic acid (2.5 mg/mL) in Tris.HCl buffer (50 mM), pH 8.0 at 40°C.