Phoenixin-20 (TFA) (PNX-20 (TFA)) is a bioactive peptide with hormone-like actions in vertebrates, and can stimulates hypothalamo-pituitary-gonadal hormones and regulate reproductive processes in mammals. Phoenixin-20 (TFA) promotes neuronal mitochondrial biogenesis via CREB-PGC-1α pathway. Phoenixin-20 (TFA) has anxiolytic effect.