PKN3-IN-1 (compound 16) inhibits PKN3 (serine/threonine protein kinase 3) and GAK ( cyclin G-associated kinase ) with IC 50 of 0.014 μM and K i of 0.0044 μM respectively.PKN3-IN-1 is a potential tool compound to study the cell biology of PKN3 and its role in pancreatic and prostate cancer and T-cell acute lymphoblastic leukemia.