计算溶液所需的质量、体积或浓度。
蛋白 A/G IgG 结合缓冲液 (1×)
有货
为什么选择此级别
BioReagent,无菌过滤 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Aladdin Protein A/G IgG 结合缓冲液 (1×) 是一款即用型磷酸盐缓冲液，专为使用Protein A、Protein G或Protein A/G亲和介质进行高产量、非变性抗体纯化而设计。该经精心优化的溶液能确保最大抗体结合容量，同时保持纯化免疫球蛋白的结构完整性和生物活性，为科研和生产应用提供可靠性能。
主要特点
广泛兼容性：单一缓冲液兼容Protein A、Protein G及Protein A/G亲和介质，提供灵活便捷的纯化解决方案。
即用型便利：预稀释的1×溶液无需额外配制，节省时间并确保结果一致性。
保持抗体完整性：温和的非变性结合条件有助于维持纯化抗体的天然结构和生物活性。
增强结合效率：特殊优化的磷酸盐体系可促进抗体与亲和基质的高特异性结合。
严格质量控制：经过严格过滤和质量检测，确保卓越的批间一致性和可靠性。
应用领域
本产品广泛适用于多种抗体纯化场景：
纯化来自不同来源（如血清、腹水或细胞培养上清液）的IgG及其亚型，适用于包括小鼠、兔和人类在内的多种物种。
可与Protein A、Protein G或Protein A/G琼脂糖/磁珠配合，用于手动或自动化纯化流程。
推荐方案
1. 使用Protein A/G IgG结合缓冲液（1×）平衡亲和介质。
2. 将溶于结合缓冲液的样品加至预处理好的树脂。
3. 执行标准清洗步骤去除未结合杂质。
4. 使用Aladdin IgG洗脱缓冲液（提供低pH与温和高盐两种配方）洗脱纯化的IgG。
5. 为获得最佳效果，建议搭配Aladdin Protein A/G琼脂糖以实现最大结合容量。
储存与稳定性
2-8°C条件下储存；自生产日期起有效期12个月。
开封后若需长期保存，请采用无菌操作技术。
Aladdin's Protein A/G IgG Binding Buffer (1×) is a ready-to-use, phosphate-based buffer, specifically formulated for high-yield, nondenaturing antibody purification using Protein A, Protein G, or Protein A/G affinity supports. This carefully optimized solution ensures maximum antibody binding capacity while maintaining the structural integrity and biological activity of purified immunoglobulins, providing reliable performance for both research and production applications.
Key Features
Universal Compatibility: A single buffer compatible with Protein A, Protein G, and Protein A/G affinity media, providing a flexible and convenient purification solution.
Ready-to-Use Convenience: Pre-diluted 1× solution requires no additional preparation, saving time and ensuring consistent results
Preserves Antibody Integrity: The gentle, nondenaturing binding conditions help maintain the native structure and biological activity of purified antibodies.
Enhanced Binding Efficiency: Specially formulated phosphate system promotes high-specificity antibody binding to affinity matrices
Rigorous Quality Control: Undergoes strict filtration and quality testing to ensure exceptional batch-to-batch consistency and reliability.
Applications
This product is widely used in various antibody purification scenarios:
Purification of IgG and its subtypes from diverse sources such as serum, ascites fluid, or cell culture supernatants from multiple species including mouse, rabbit, and human.
Compatible with Protein A, Protein G, or Protein A/G agarose/magnetic beads for manual or automated purification protocol.
Recommended Protocol
1. Equilibrate affinity support with Protein A/G IgG Binding Buffer (1×).
2. Apply sample in binding buffer to the prepared resin.
3. Perform standard wash steps to remove unbound contaminants.
4. Elute purified IgG using Aladdin's IgG Elution Buffer (available in both low-pH and gentle high-salt formulations).
5. For optimal results, pair with Aladdin's Protein A/G Agarose for maximum binding capacity.
Storage & Stability
Store at 2-8°C; stable for 12 months from manufacture date.
Use aseptic techniques for extended storage of opened containers.
规格
别名
结合缓冲液
英文别名
Binding Buffer
规格或纯度
无菌过滤, BioReagent
稳定性与储存
Store at 2-8 ℃ long term (12 months).
英文名称
Protein A/G IgG Binding Buffer (1×)
储存条件
2-8°C储存
运输条件
低温运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
技术文档和文章
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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