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Plus血浆低丰度蛋白富集及前处理试剂盒
有货
为什么选择此级别
BioReagent,无菌,蛋白组学级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光,室温,-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
本产品为从血浆到肽段的一站式处理试剂盒，采用纳米技术有效富集血浆低丰度蛋白，结合“All In One Tube”创新理念处理血浆蛋白，即便是没有组学背景的科研人员，也可以快速的制备前处理的样品，可直接用于后续的质谱分析。
产品组分及储存条件：
| 项目号 | Component | 10T | 25T | Storage |
| P1456360A | Magnetic beads | 10T | 25T | 4℃ |
| P1456360B | Incubation Buffer I | 4.8 mL | 12 mL | RT |
| P1456360C | Incubation Buffer II | 6 mL | 15 mL | RT |
| P1456360D | Washing Buffer | 6 mL | 15 mL | RT |
| P1456360E | Lysis Buffer | 0.6 mL | 1.5 mL | -20℃. Store in the dark. |
| P1456360F | Balance Buffer | 2.8mL | 7 mL | 4℃ |
| P1456360G | Digest Buffer | 16 μL | 40 μL | -20℃ |
| P1456360H | Stop Buffer | 300 μL | 750 μL | RT |
| P1456360I | Wash Buffer I | 4 mL | 10 mL | RT |
| P1456360J | Wash Buffer II | 2.4 mL | 6 mL | RT |
| P1456360K | Wash Buffer III | 2.4 mL | 6 mL | RT |
| P1456360L | Elution Buffer | 4.8 mL | 12 mL | RT. Store in the dark. |
| P1456360M | Loading Buffer | 120 μL | 300 μL | RT |
| P1456360N | Tip柱 | 10T | 25T | RT |
产品特点：
方便快捷：从血浆到肽段的处理，一盒搞定。
操作简单：使用仪器简单，仅需常规离心机及金属浴。
稳定性好：每批次经过严格质检，实验结果重复性高。
操作流程：
1.血浆样本离心 (3000g，10min)，取上清备用 (如需保存请置于-80°C条件下长期保存，避免反复冻融)。
2.取50-100μL离心后的血浆，加入400μL的Incubation buffer I，再加入30μL的Magnetic beads，涡旋混匀，置于震荡混匀仪室温孵育1h。
3.孵育结束后，用磁力架磁吸3min，弃上清。
4.取下Ep管，加入500μL Incubation buffer II，轻柔上下颠倒混匀多次，磁吸3min，弃上清。
5.取下Ep管，加入500μL Washing Buffer，轻柔上下颠倒混匀多次，磁吸3min，弃上清。
6.取下Ep管，加入50μL lysis Buffer，重悬beads，水浴，95°C，10min，然后取出并冷却到室温。
7.向冷却至室温的EP管中加入225μL的Balance bufer。
8.向样品中加入1μL的Digest buffer，37°C，1200rpm金属浴震荡酶解3-16h。
9.酶解结束后取出EP管，向样品中加入25μL的Stop buffer，涡旋混匀。
10.再加入320μL Wash buffer I，剧烈震荡3min，15000rpm离心3min，将上层液体去除。
11.将下层样品转入Tip柱中，2500rpm，离心3-5min，直到液体全部离心下来，如果液体流速较慢，可以适当加大转速。
12.向脱盐柱中加入200μL的Wash buffer II (用之前震荡10-20s)，2500rpm，离心3-5min，直到液体全部离心下来。
13.向脱盐柱中加入200μL的Wash buffer III，2500rpm/min，离心3-5 min，直到液体全部离心下来。
14.将脱盐柱重新放入一个新的EP管中，向脱盐柱中加入200μL的Elution buffer，2000rpm，离心3-5min，直到液体全部离心下来。
15.重复上一步骤，收集两次的洗脱液，冷冻干燥。
16.加入10μL的Loading buffer，剧烈涡匀3min，离心2000g、10min，取适量样品，即可质谱检测。以HF-X仪器为例，上样0.5-1μg即可。
自动化操作流程：
适配质谱蛋白质组前处理工作站，从血浆到肽段的一站式处理，消除人工操作误差，提高样本制备工作流程的可重复性，为各种实验室流程提供高精确度和可靠性。
Product Introduction:
This product is a one-stop processing kit from plasma to peptides. It utilizes nanotechnology to effectively enrich low-abundance proteins in plasma and processes plasma proteins in combination with the innovative concept of "All In One Tube". Even researchers without omics background can quickly prepare pretreated samples, which can be directly used for subsequent mass spectrometry analysis.
Product Components and Storage Conditions:
| P1456360 | Component | 10T | 25T | Storage |
| P1456360A | Magnetic beads | 10T | 25T | 4℃ |
| P1456360B | Incubation Buffer I | 4.8 mL | 12 mL | RT |
| P1456360C | Incubation Buffer II | 6 mL | 15 mL | RT |
| P1456360D | Washing Buffer | 6 mL | 15 mL | RT |
| P1456360E | Lysis Buffer | 0.6 mL | 1.5 mL | -20℃. Store in the dark. |
| P1456360F | Balance Buffer | 2.8mL | 7 mL | 4℃ |
| P1456360G | Digest Buffer | 16 μL | 40 μL | -20℃ |
| P1456360H | Stop Buffer | 300 μL | 750 μL | RT |
| P1456360I | Wash Buffer I | 4 mL | 10 mL | RT |
| P1456360J | Wash Buffer II | 2.4 mL | 6 mL | RT |
| P1456360K | Wash Buffer III | 2.4 mL | 6 mL | RT |
| P1456360L | Elution Buffer | 4.8 mL | 12 mL | RT. Store in the dark. |
| P1456360M | Loading Buffer | 120 μL | 300 μL | RT |
| P1456360N | Tip柱 | 10T | 25T | RT |
Product Features:
Convenient and fast: The processing from plasma to peptides can be completed with one kit.
Simple operation: The instruments used are simple, requiring only a conventional centrifuge and a metal bath.
Good stability: Each batch undergoes strict quality inspection, ensuring high reproducibility of experimental results.
Operating Procedure:
1.Centrifuge the plasma sample (3000g, 10min), and take the supernatant for later use (if storage is required, store it at -80°C for long-term preservation to avoid repeated freezing and thawing).
2.Take 50-100μL of centrifuged plasma, add 400μL of Incubation buffer I, then add 30μL of Magnetic beads, vortex to mix, and incubate at room temperature on a shaking mixer for 1 hour.
3.After incubation, use a magnetic rack to magnetically separate for 3min, and discard the supernatant.
4.Remove the EP tube, add 500μL of Incubation buffer II, gently invert up and down to mix several times, magnetically separate for 3min, and discard the supernatant.
5.Remove the EP tube, add 500μL of Washing Buffer, gently invert up and down to mix several times, magnetically separate for 3min, and discard the supernatant.
6.Remove the EP tube, add 50μL of Lysis Buffer to resuspend the beads, place in a water bath at 95°C for 10min, then take it out and cool to room temperature.
7.Add 225μL of Balance buffer to the EP tube that has cooled to room temperature.
8.Add 1μL of Digest buffer to the sample, and perform enzymatic digestion with shaking in a metal bath at 37°C and 1200rpm for 3-16h.
9.After enzymatic digestion, take out the EP tube, add 25μL of Stop buffer to the sample, and vortex to mix.
10.Add 320μL of Wash buffer I, shake vigorously for 3min, centrifuge at 15000rpm for 3min, and remove the upper liquid.
11.Transfer the lower layer sample into the Tip column, centrifuge at 2500rpm for 3-5min until all the liquid is centrifuged down. If the liquid flow rate is slow, the rotation speed can be appropriately increased.
12.Add 200μL of Wash buffer II (shake for 10-20s before use) to the desalting column, centrifuge at 2500rpm for 3-5min until all the liquid is centrifuged down.
13.Add 200μL of Wash buffer III to the desalting column, centrifuge at 2500rpm for 3-5min until all the liquid is centrifuged down.
14.Put the desalting column into a new EP tube, add 200μL of Elution buffer to the desalting column, centrifuge at 2000rpm for 3-5min until all the liquid is centrifuged down.
15.Repeat the previous step, collect the eluates from both times, and freeze-dry them.
16.Add 10μL of Loading buffer, vortex vigorously for 3min, centrifuge at 2000g for 10min, take an appropriate amount of sample for mass spectrometry detection. Taking the HF-X instrument as an example, 0.5-1μg of sample is sufficient for loading.
Automated Operation Process:
It is compatible with mass spectrometry proteomics pretreatment workstations, enabling one-stop processing from plasma to peptides. This eliminates manual operation errors, improves the reproducibility of sample preparation workflows, and provides high precision and reliability for various laboratory procedures.
规格
别名
血浆低丰度蛋白富集及前处理试剂盒 | 低丰度蛋白富集及前处理试剂盒
英文别名
Low Abundance Protein Enrichment and Pretreatment Kit
规格或纯度
BioReagent, 无菌, 蛋白组学级
英文名称
Plus Plasma Low Abundance Protein Enrichment and Pretreatment Kit
应用
低丰度蛋白富集, 蛋白质组样品前处理, 质谱分析
敏感性
Light-sensitive
储存与运输
储存条件
2-8°C储存,避光,室温,-20°C储存
运输条件
超低温运输
稳定性与储存
P1456360A、P1456360F: Store at 2-8℃ long term (12 months). P1456360E: Store at -20℃ long term (12 months). Store in the dark. P1456360G: Store at -20℃ long term (12 months). P1456360L: Store at room temperature long term (12 months). Store in the dark. P14
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ25F0927201 |
分析证书 | P1456360 |
技术文档和文章
溶液计算器
常见问题
什么是「蛋白组学级」？
「蛋白组学级」表示该产品经过加工和检测，适用于分子生物学和蛋白质组学。典型规格包括：低或不可检测的核酸酶/蛋白酶活性、低内毒素、受控的微生物限度，以及高批间重现性。
什么是「BioReagent」？
「BioReagent」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
本产品应如何储存？
请于?20 °C、避光条件下保存。本品对光敏感，请保存在原始的避光或棕色容器中。
本产品如何运输？
本产品采用保温箱配冰袋运输。收货后请立即拆包，并按上述储存条件保存。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
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