计算溶液所需的质量、体积或浓度。
磷酸肽蛋白（肽段）提取试剂盒
有货
为什么选择此级别
BioReagent,无菌,蛋白组学级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,室温。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
由于磷酸化的化学计量比较低，因此磷酸肽的浓缩是成功进行磷酸蛋白质组学实验的关键步骤。PolyMAC提供了一种有效的、大大改进的方法来实现更完整的磷酸肽的富集化。这种高度选择性的富集法可以用于大多数复杂样品，因为它提供了最佳的专属性和回收率。PolyMAC是一种基于聚合物的固定化金属离子亲和捕获法，具有卓越的磷酸化蛋白质组覆盖率和回收率。它是一种可溶性纳米聚合物，设计用于与溶液中的磷肽相互作用，随后在固相载体上捕获，用于洗涤和磷肽洗脱。与常用的二氧化钛和固定化金属离子亲和捕捉法相比，PolyMAC具有更好的重现性和磷酸化肽段的富集性，磷酸化肽段的选择性接近95%，回收率>90%。重要的是，对于总蛋白磷酸化水平较低的样品，PolyMAC具有非常好的回收率和选择性。
磷酸肽蛋白（肽段）提取试剂盒（P1456460）实验流程图
产品组分及储存条件：
| 项目号 | Component | 24T | 48T | 96T | Storage |
| P1456460A | PolyMAC Beads | 0.75 mL | 1.5 mL | 3 mL | 4℃ |
| P1456460B | PolyMAC Loading Buffer | 25 mL | 50 mL | 100 mL | RT |
| P1456460C | PolyMAC Washing Buffer I | 25 mL | 50 mL | 100 mL | RT |
| P1456460D | PolyMAC Washing Buffer II | 25 mL | 50 mL | 100 mL | RT |
| P1456460E | PolyMAC Elution Buffer | 3.75 mL | 7.5 mL | 15 mL | RT |
| P1456460F | PolyMAC Tips | 24T | 48T | 96T | RT |
产品特点：
操作简单：可快速制备磷酸肽，仅需一台金属浴和常规离心机
稳定性高：各批次严格质检，实验结果重复性高
操作流程：
1.向酶解和脱盐后的干燥样品 (50μg-100μg) 中加入200μL的Loading Buffer充分重悬样品。
2.将PolyMAC Beads充分涡旋 (10-20s) 后取25μL于1.5mL的离心管中，瞬离2-3s，然后去除上层储存液。(Beads 沉降较快，取用时需动作迅速)
3.将重悬后的样品加入到含有PolyMAC Beads的离心管中，然后置于混匀仪中，26℃，>1200rpm，剧烈摇晃25min。
4.将样品加入PolyMAC-Tips中，先20g离心2min，然后100g离心1min，确保样品溶液从Tips尖端流出到离心管中。(转速不宜过大，且动作迅速)
5.用200μL Loading Buffer 洗1次，先20g离心2 min，然后100g离心1min，弃滤液。重复3次。(转速不宜过大，且动作迅速)
6.用200μL Washing Buffer I洗1次，先20g离心2min，然后100g离心1min，弃滤液。重复3次。(转速不宜过大，且动作迅速)
7.用200μL Washing Buffer lI洗1次，先20g离心2min，然后100g离心1min，弃滤液。重复3次。(转速不宜过大，且动作迅速)
8.将Tips放入新离心管中以收集洗脱的磷酸肽，Tips中加入50μL Elution Bufer，20g离心2min，再加入50μL Elution Buffer，先20g离心2min，然后100g离心1min，收集滤液。(转速不宜过大，且动作迅速)
注：4-8步骤，液体下流速度较慢时，转速可适当增加，50g逐步增加，切勿一次增加太多!
9.如果有任何溶液残留在吸头中，请用移液器将其推出收集管中，冻干后-80℃保存待质谱检测。
注意事项：
本产品仅限于专业人员的科学研究使用，不得用于临床诊断或治疗，不得用于食品或药品。
Product Introduction:
Due to the low stoichiometry of phosphorylation, the concentration of phosphopeptides is a key step for the successful implementation of phosphoproteomics experiments. PolyMAC provides an effective and significantly improved method to achieve more complete enrichment of phosphopeptides. This highly selective enrichment method can be used for most complex samples because it offers optimal specificity and recovery rate. PolyMAC is a polymer-based immobilized metal ion affinity capture method with excellent phosphoproteome coverage and recovery rate. It is a soluble nanopolymer designed to interact with phosphopeptides in solution, and then be captured on a solid-phase carrier for washing and phosphopeptide elution. Compared with the commonly used titanium dioxide and immobilized metal ion affinity capture methods, PolyMAC has better reproducibility and enrichment of phosphorylated peptides, with the selectivity of phosphorylated peptides close to 95% and the recovery rate > 90%. Importantly, PolyMAC has very good recovery rate and selectivity for samples with low total protein phosphorylation levels.
Experimental Flowchart of Phosphopeptide Protein (Peptide Fragment) Extraction Kit (P1456460)
Product Components and Storage Conditions:
| P1456460 | Component | 24T | 48T | 96T | Storage |
| P1456460A | PolyMAC Beads | 0.75 mL | 1.5 mL | 3 mL | 4℃ |
| P1456460B | PolyMAC Loading Buffer | 25 mL | 50 mL | 100 mL | RT |
| P1456460C | PolyMAC Washing Buffer I | 25 mL | 50 mL | 100 mL | RT |
| P1456460D | PolyMAC Washing Buffer II | 25 mL | 50 mL | 100 mL | RT |
| P1456460E | PolyMAC Elution Buffer | 3.75 mL | 7.5 mL | 15 mL | RT |
| P1456460F | PolyMAC Tips | 24T | 48T | 96T | RT |
Product Features:
Easy to operate: Phosphopeptides can be prepared quickly, requiring only a metal bath and a conventional centrifuge.
High stability: Strict quality inspection is conducted for each batch, ensuring high reproducibility of experimental results.
Operating Procedure:
1.Add 200 μL of Loading Buffer to the dried sample (50 μg - 100 μg) after enzymatic hydrolysis and desalting, and fully resuspend the sample.
2.Vortex the PolyMAC Beads thoroughly (for 10 - 20 seconds), then take 25 μL into a 1.5 mL centrifuge tube, centrifuge instantaneously for 2 - 3 seconds, and then remove the upper storage solution. (The Beads settle quickly, so the operation should be fast when taking them.)
3.Add the resuspended sample to the centrifuge tube containing PolyMAC Beads, then place it on a mixer, and shake vigorously at 26°C, >1200 rpm for 25 minutes.
4.Add the sample to PolyMAC - Tips, first centrifuge at 20 g for 2 minutes, then centrifuge at 100 g for 1 minute to ensure that the sample solution flows out from the tip of the Tips into the centrifuge tube. (The rotation speed should not be too high, and the operation should be fast.)
5.Wash once with 200 μL of Loading Buffer: first centrifuge at 20 g for 2 minutes, then centrifuge at 100 g for 1 minute, and discard the filtrate. Repeat this step 3 times. (The rotation speed should not be too high, and the operation should be fast.)
6.Wash once with 200 μL of Washing Buffer I: first centrifuge at 20 g for 2 minutes, then centrifuge at 100 g for 1 minute, and discard the filtrate. Repeat this step 3 times. (The rotation speed should not be too high, and the operation should be fast.)
7.Wash once with 200 μL of Washing Buffer II: first centrifuge at 20 g for 2 minutes, then centrifuge at 100 g for 1 minute, and discard the filtrate. Repeat this step 3 times. (The rotation speed should not be too high, and the operation should be fast.)
8.Put the Tips into a new centrifuge tube to collect the eluted phosphopeptides. Add 50 μL of Elution Buffer to the Tips, centrifuge at 20 g for 2 minutes, then add another 50 μL of Elution Buffer, first centrifuge at 20 g for 2 minutes, then centrifuge at 100 g for 1 minute, and collect the filtrate. (The rotation speed should not be too high, and the operation should be fast.)
Note: In steps 4 - 8, if the liquid flows down slowly, the rotation speed can be increased appropriately, gradually increasing to 50 g, and do not increase too much at one time!
9.If there is any solution remaining in the pipette tip, push it out into the collection tube with a pipette, lyophilize it, and store it at -80°C for mass spectrometry detection.
Precautions:
This product is only for scientific research use by professionals, and shall not be used for clinical diagnosis or treatment, nor for food or drugs.
规格
别名
磷酸肽蛋白提取试剂盒
英文别名
Phosphopeptide Protein Extraction Kit
规格或纯度
BioReagent, 无菌, 蛋白组学级
英文名称
Phosphopeptide Protein (Peptide Fragment) Extraction Kit
应用
磷酸肽富集, 蛋白质组样品前处理, 质谱分析
储存与运输
储存条件
2-8°C储存,室温
运输条件
低温运输
稳定性与储存
P1456460A: Store at 2-8℃ long term (12 months). P1456460B、P1456460C、P1456460D、P1456460E、P1456460F: Store at room temperature long term (12 months).
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ25F0926928 |
分析证书 | P1456460 |
| ZJ26F0535476 |
分析证书 | P1456460 |
| ZJ25F0926929 |
分析证书 | P1456460 |
技术文档和文章
溶液计算器
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