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蛋白组学前处理试剂盒
有货
为什么选择此级别
BioReagent,无菌,蛋白组学级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光,室温,-20°C储存。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
目前常规的蛋白质组样品前处理过程存在着诸多问题，如重现性差、方法稳定性不好、灵敏度不高且非常耗时及无法自动化等。 针对上述问题，阿拉丁推出新一代“All In One Tube”的创新产品，本产品主要是针对微量蛋白（5-100μg）的处理，即便是没有组 学背景的科研人员，也可以快速的制备前处理的样品，可用于后续的质谱分析。
蛋白组学前处理试剂盒（P1456469）实验流程图
产品组分及储存条件：
| P1456469 | Component | 12 T | 24T | 48T | Storage |
| P1456469A | Lysis Buffer | 1.25 mL | 2.5 mL | 5 mL | -20℃. Store in the dark. |
| P1456469B | Digest | 0.05 mL | 0.1 mL | 0.2 mL | -20℃ |
| P1456469C | Balance Buffer | 3 mL | 6 mL | 12 mL | 2-8℃ |
| P1456469D | Stop Buffer | 0.375 mL | 0.75 mL | 1.5 mL | RT |
| P1456469E | Wash Buffer I | 5 mL | 10 mL | 20 mL | RT |
| P1456469F | Wash Buffer II | 3.75 mL | 7.5 mL | 15 mL | RT |
| P1456469G | Wash Buffer III | 3.75 mL | 7.5 mL | 15 mL | RT |
| P1456469H | Elution Buffer | 6.25 mL | 12.5 mL | 25 mL | RT. Store in the dark. |
| P1456469I | Loading Buffer | 0.25 mL | 0.5 mL | 1 mL | RT |
| P1456469J | Tip柱 | 12 T | 24T | 48T | RT |
产品特点：
微量蛋白：针对5-100μg的蛋白前处理
操作简单：可快速制备前处理样品，仅需一台金属浴和常规离心机
稳定性高：各批次严格质检，实验结果重复性高
操作流程：
1.裂解
①取50μL的Lysis buffer加入到含有样品的EP管中，震荡混匀。
②将含有Lysis buffer的EP管水浴95°C、10min，然后取出冷却至室温。
2.酶解
①向冷却至室温的EP管中加入225μL的Balance buffer。
②加入3μL的Digest后混匀，37°C，1200rpm， 震荡酶解，过夜(建议16h)。
3.除盐(室温)
①向样品中加入25μL的Stop buffer，涡旋混匀。
②再加入320μL Wash buffer 1,剧烈震荡3min，15000rpm离心3min，将上层液体去除。
③将下层样品转入Tip柱中，2500rpm/min,离心3-5min，直到液体全部离心下来，如果液体流速较慢，可以加大转速。
④向脱盐柱中加入200μL的Wash buffer 2 (用之前震荡10-20s)，2500rpm/min，离心3-5min，直到液体全部离心下来。
⑤向脱盐柱中加入200μL的Wash buffer 3，2500rpm/min，离心3-5min，直到液体全部离心下来。
⑥将脱盐柱重新放入一个新的EP管中，向脱盐柱中加入200μL的Elution buffer，2000rpm/min，离心3-5min，直到液体全部离心下来。
⑦重复步骤⑥，收集两次的洗脱液，冷冻干燥。
⑧加入10μL的Loading buffer，剧烈涡匀3min，离心20000g、10min，取适量样品，即可质谱检测。以HF-X仪器为例，上样0.5-1μg即可。
注意事项：
1. Digest分装后-20°C保存；
2. Lysis Buffer分装后-20°C保存，避免反复冻融；
3.本产品仅限于专业人员的科学研究使用，不得用于临床诊断或治疗，不得用于食品或药品。
Product Introduction:
At present, the conventional proteome sample pretreatment process has many problems, such as poor reproducibility, poor method stability, low sensitivity, being very time-consuming, and inability to be automated. To address the above issues, Aladdin has launched a new generation of "All In One Tube" innovative products. This product is mainly designed for the processing of trace proteins (5-100μg). Even researchers without omics background can quickly prepare pretreated samples, which can be used for subsequent mass spectrometry analysis.
Experimental Flowchart of Proteomics Pretreatment Kit (P1456469)
Product Components and Storage Conditions:
| P1456469 | Component | 12 T | 24T | 48T | Storage |
| P1456469A | Lysis Buffer | 1.25 mL | 2.5 mL | 5 mL | -20℃. Store in the dark. |
| P1456469B | Digest | 0.05 mL | 0.1 mL | 0.2 mL | -20℃ |
| P1456469C | Balance Buffer | 3 mL | 6 mL | 12 mL | 2-8℃ |
| P1456469D | Stop Buffer | 0.375 mL | 0.75 mL | 1.5 mL | RT |
| P1456469E | Wash Buffer I | 5 mL | 10 mL | 20 mL | RT |
| P1456469F | Wash Buffer II | 3.75 mL | 7.5 mL | 15 mL | RT |
| P1456469G | Wash Buffer III | 3.75 mL | 7.5 mL | 15 mL | RT |
| P1456469H | Elution Buffer | 6.25 mL | 12.5 mL | 25 mL | RT. Store in the dark. |
| P1456469I | Loading Buffer | 0.25 mL | 0.5 mL | 1 mL | RT |
| P1456469J | Tip pillar | 12 T | 24T | 48T | RT |
Trace proteins: Designed for the pretreatment of 5-100μg proteins.
Simple operation: Enables rapid preparation of pretreated samples, requiring only a metal bath and a conventional centrifuge.
High stability: Strict quality inspection for each batch ensures high reproducibility of experimental results.
Operating Procedure:
Lysis
① Add 50μL of Lysis buffer to the EP tube containing the sample and mix by shaking.
② Place the EP tube with Lysis buffer in a 95°C water bath for 10 minutes, then take it out and cool to room temperature.
Enzymatic Digestion
① Add 225μL of Balance buffer to the EP tube that has cooled to room temperature.
② Add 3μL of Digest, mix well, and perform enzymatic digestion with shaking at 37°C and 1200rpm overnight (16 hours is recommended).
Desalting (at room temperature)
① Add 25μL of Stop buffer to the sample and vortex to mix.
② Add 320μL of Wash buffer 1, shake vigorously for 3 minutes, centrifuge at 15000rpm for 3 minutes, and remove the supernatant.
③ Transfer the lower layer sample into the Tip column, centrifuge at 2500rpm for 3-5 minutes until all liquid is centrifuged down. If the liquid flow rate is slow, the speed can be increased.
④ Add 200μL of Wash buffer 2 (shake for 10-20 seconds before use) to the desalting column, centrifuge at 2500rpm for 3-5 minutes until all liquid is centrifuged down.
⑤ Add 200μL of Wash buffer 3 to the desalting column, centrifuge at 2500rpm for 3-5 minutes until all liquid is centrifuged down.
⑥ Put the desalting column into a new EP tube, add 200μL of Elution buffer to the desalting column, centrifuge at 2000rpm for 3-5 minutes until all liquid is centrifuged down.
⑦ Repeat step ⑥, collect the eluates from both times, and freeze-dry them.
⑧ Add 10μL of Loading buffer, vortex vigorously for 3 minutes, centrifuge at 20000g for 10 minutes, take an appropriate amount of sample, and then mass spectrometry detection can be performed. Taking the HF-X instrument as an example, 0.5-1μg of sample is sufficient for loading.
Precautions:
After aliquoting, Digest should be stored at -20°C.
After aliquoting, Lysis Buffer should be stored at -20°C and avoid repeated freezing and thawing.
This product is limited to scientific research use by professionals, and must not be used for clinical diagnosis or treatment, nor for food or drugs.
规格
规格或纯度
BioReagent, 无菌, 蛋白组学级
英文名称
Proteomic Pretreatment Kit
应用
蛋白质组样品前处理, 质谱分析
敏感性
Light-sensitive
储存与运输
储存条件
2-8°C储存,避光,室温,-20°C储存
运输条件
低温运输
稳定性与储存
各组分在相应的储存条件下保质期1年。
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ25F0927612 |
分析证书 | P1456469 |
| ZJ25F0926874 |
分析证书 | P1456469 |
技术文档和文章
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