计算溶液所需的质量、体积或浓度。
,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
是否无菌:是
本产品是一种专为Protein A、Protein G、Protein A/G抗体纯化柱料的洗脱而设计的缓冲液。其低pH环境(pH 2.5-3.0)能够有效破坏Protein A/G与IgG抗体Fc段之间的高亲和力相互作用,从而将抗体从柱料上解离下来,实现高效洗脱。本缓冲液适用于纯化多种物种来源(如人、兔、小鼠、大鼠等)的多克隆或单克隆抗体。
使用时需用去离子水将5x缓冲液稀释至1x,具体使用方法可根据不同纯化工具进行使用。
This product is a buffer specifically designed for the elution of antibodies from Protein A, Protein G, and Protein A/G purification columns. Its low-pH environment (pH 2.5-3.0) effectively disrupts the high-affinity interaction between Protein A/G and the Fc region of IgG antibodies, thereby dissociating the antibodies from the column matrix and achieving efficient elution. This buffer is suitable for purifying polyclonal or monoclonal antibodies derived from various species, such as human, rabbit, mouse, rat, and others.
Prepare 1X Protein A/G Elution Buffer by diluting Protein Protein A/G Buffer(5X) with deionized water. The specific method of use may vary depending on the purification tool employed.
| pH | pH 2.8±0.3 |
|---|
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | P1492569 |