计算溶液所需的质量、体积或浓度。
蛋白组学前处理试剂盒
有货
为什么选择此级别
BioReagent,for MS,用于蛋白分析,蛋白组学级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温,-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
本试剂盒是专为基于质谱的抗体药物表征与质量控制而设计的一体化、高性能样品前处理解决方案。它通过优化的流程，将复杂的抗体蛋白（如单克隆抗体、双特异性抗体等）高效、稳定地酶解为肽段混合物，为后续的肽图分析奠定坚实基础。该分析是确保抗体药物一级结构准确性、鉴定关键质量属性（如翻译后修饰）的核心技术。本产品主要是针对微量蛋白、抗体（5-100 μg）的处理，即便是没有组学背景的科研人员，也可以快速的制备前处理的样品，可用于后续的质谱分析。
| P1501114A | Components | 10T | 100T | Storage | Quantity Per Test |
| P1501114A | Denaturation Buffer | 0.5 mL | 5 mL | RT | 50μL per 5-100 μg sample |
| P1501114B | Reduction Buffer | 10 μL | 100 μL | -20℃. Store in the dark. | 1μL per 5-100 μg sample |
| P1501114C | Alkylation Buffer | 30 μL | 300 μL | -20℃. Store in the dark. | 3μL per 5-100 μg sample |
| P1501114D | Stop Buffer | 1 mL | 10 mL | RT | 100μL per 5-100 μg sample |
注: 每个Test推荐样品质量为 5-100 μg
Note: The recommended sample mass for each test is 5-100 μg.
注意事项：
1.Reduction Buffer分装后-20 ℃保存 ，避免反复冻融；
2. Alkylation Buffer 分装后-20 ℃保存，避免反复冻融；
3. 本产品不提供酶及超滤离心管，用户可根据需求自行选择；
4. 本产品仅限于专业人员的科学研究使用，不得用于临床诊断或治疗，不得用于食品或药品。
使用说明：
1.取一定量样品 ，加入50μL的Denaturation Buffer，混匀，待用。
2.每份样品中加入2μL的Reduction Buffer，混匀后于56℃反应30min。
3. 每份样品中加入3μL的Alkylation Buffer，混匀后于室温下避光放置 30分钟。
4.换液
每份样品分别转移至合适分子量的超滤离心管中，10000rpm，60分钟(可根据实际情况适当调整离心时间至离干为止)，用对应的酶解缓冲液混匀，待用。
5. 酶解
本产品不提供酶，用户需根据实验需求进行酶解。
6.终止反应
酶解完毕，每份样品中加入100μLStop Buffer，转移至进样小瓶中备用。
This kit is an all-in-one, high-performance sample preparation solution specifically designed for mass spectrometry-based characterization and quality control of antibody drugs. Through an optimized workflow, it efficiently and stably digests complex antibody proteins (such as monoclonal antibodies, bispecific antibodies, etc.) into peptide mixtures, laying a solid foundation for subsequent peptide mapping analysis. This analysis is a core technique for ensuring the accuracy of the primary structure of antibody drugs and identifying critical quality attributes (such as post-translational modifications). The product is primarily designed for processing low amounts of proteins and antibodies (5–100 μg), allowing researchers, even those without an omics background, to quickly prepare preprocessed samples for subsequent mass spectrometry analysis.
| P1501114A | Components | 10T | 100T | Storage | Quantity Per Test |
| P1501114A | Denaturation Buffer | 0.5 mL | 5 mL | RT | 50μL per 5-100 μg sample |
| P1501114B | Reduction Buffer | 10 μL | 100 μL | -20℃. Store in the dark. | 1μL per 5-100 μg sample |
| P1501114C | Alkylation Buffer | 30 μL | 300 μL | -20℃. Store in the dark. | 3μL per 5-100 μg sample |
| P1501114D | Stop Buffer | 1 mL | 10 mL | RT | 100μL per 5-100 μg sample |
注: 每个Test推荐样品质量为 5-100 μg
Note: The recommended sample mass for each test is 5-100 μg.
Precautions
1.Aliquot and store the Reduction Buffer at -20°C, and avoid repeated freeze-thaw cycles.
2.Aliquot and store the Alkylation Buffer at -20°C, and avoid repeated freeze-thaw cycles.
3.Enzymes and ultrafiltration centrifuge tubes are not provided with this product. Users may select them according to their needs.
4.This product is intended for scientific research use by professionals only. It is not to be used for clinical diagnosis or treatment, nor for food or drug purposes.
Instruction for use
1.Take a certain amount of sample add 50 μL of Denaturation Buffer and mix well. Set aside for use.
2.Add 1 μL of Reduction Buffer to each sample. Mix well and react at 56 °C for 30 min.
3.Add 3 μL of Alkylation Buffer to each sample. Mix well and incubate at room temperature in the dark for 30 minutes.
4.Buffer Exchange
Transfer each sample into an ultrafiltration centrifuge tube with an appropriate molecular weight cut-off. Centrifuge at 10,000 rpm for 60 minutes (the centrifugation time can be adjusted appropriately according to the actual situation until the solution has passed through). Mix with the corresponding digestion buffer and set aside for use.
5.Digestion
Enzymes are not provided with this product. Users should perform the digestion according to their experimental requirements.
6.Reaction Termination
After digestion is complete, add 100 μL of Stop Buffer to each sample. Transfer the mixture into an injection vial for later use.
规格
别名
蛋白组学样本前处理试剂盒
英文别名
MS based Proteomics Sample Preparation Kit | Proteomics sample pretreatment kit
规格或纯度
BioReagent,用于蛋白分析,蛋白组学级,for MS
英文名称
Proteomics Sample Preparation Kit
应用
蛋白质组样品前处理, 质谱分析
敏感性
Light-sensitive
储存与运输
储存条件
避光,室温,-20°C储存
运输条件
超低温运输
组分与储存
| P1501114A | Components | 10T | 100T | Storage | Quantity Per Test |
| P1501114A | 变性缓冲液 | 0.5 mL | 5 mL | RT | 50μL per 5-100 μg sample |
| P1501114B | 还原缓冲液 | 10 μL | 100 μL | -20℃. Store in the dark. | 1μL per 5-100 μg sample |
| P1501114C | 烷基化缓冲液 | 30 μL | 300 μL | -20℃. Store in the dark. | 3μL per 5-100 μg sample |
| P1501114D | 终止液 | 1 mL | 10 mL | RT | 100μL per 5-100 μg sample |
注: 每个Test推荐样品质量为 5-100 μg
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0333601 |
分析证书 | P1501114 |
| ZJ26F0333599 |
分析证书 | P1501114 |
| ZJ26F0333597 |
分析证书 | P1501114 |
| ZJ26F0333595 |
分析证书 | P1501114 |
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