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磷脂染色液 (酸性苏木红法)
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
脂质 (Lipid)是中性脂肪、类脂及其衍生物的总称，其共同的物理特性是不溶于水，易溶于有机溶剂 (如乙醇、乙醚等)，人体的脂肪主要有两种：1、储存脂肪，如中性脂肪，主要分布于皮下、肾、胰腺等部位；2、结构脂肪，如类脂 (磷脂、糖脂、胆固醇等)，主要分布于细胞内，磷脂 (phospholipid)由甘油、脂肪酸和含氮的碱基组成，是细胞膜和细胞内膜结构的主要组成成分，磷脂在大脑、神经组织、骨髓和肝脏等组织中含量较多，磷脂可分为卵磷脂、脑磷脂和神经鞘磷脂三种，显示磷脂的常用方法有酸性苏木红法、吡啶提取法。
磷脂染色液 (酸性苏木红法)又称Baker磷脂染色液，采用甲醛钙固定组织能使磷脂受钙的作用阻留不能进入固定液，经Baker媒染液处理，使磷脂与金属离子结合成不溶性的磷脂螯合物，经酸性苏木红染色进而形成蓝黑色的复合物，磷脂、神经鞘磷脂呈蓝黑色。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
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自备材料：
1、蒸馏水、系列乙醇、甘油明胶、甲醛钙固定液
2、恒温箱或水浴锅
操作步骤(仅供参考)：
1、组织固定于甲醛钙固定液中16h (过夜)。
2、入Baker媒染液60℃恒温箱浸染24h，流水冲洗16h (过夜)。
3、低温恒冷切片机或半导体制冷切片机切片，切片厚度8μm。
4、切片用蒸馏水水洗，晾干。
5、切片再入Baker媒染液60℃恒温箱浸染1h，流水稍洗，蒸馏水水洗。
6、切片入酸性苏木红染色液37℃恒温箱浸染2~5h，流水冲洗。
7、切片入Baker分化液37℃恒温箱浸染0.5~2h，流水冲洗10min，蒸馏水水洗。
8、甘油明胶封固。
染色结果：
磷脂、神经鞘磷脂、核蛋白: 蓝色-黑色
细胞质: 灰黄色
阴性对照(可选)：
1、配制磷脂提取试剂，氯仿:甲醇 (2:1)或吡啶。
2、取连续组织切片用上述磷脂提取试剂室温处理1-2小时。
3、用相同方法进行磷脂染色应为阴性。
注意事项：
1、该染色法有可能产生假阳性，如有必要可用脂质提取试剂处理做阴性对照。
2、酸性苏木红染色液不可反复使用。
3、分化液可反复使用，直到黄色变淡才丢弃。
4、为了您的安全和健康，请穿实验服并戴一次性手套操作。
5、试剂开封后请尽快使用，以防影响后续实验效果。
Lipids are a general term for neutral fats, lipids, and their derivatives. Their common physical property is insolubility in water and solubility in organic solvents (e.g., ethanol, ether, etc.). There are two main types of fats in the human body: Storage fats (e.g., neutral fats), mainly distributed in the subcutaneous tissue, kidneys, pancreas, and other parts; Structural fats (e.g., lipids including phospholipids, glycolipids, cholesterol, etc.), mainly distributed inside cells. Phospholipids are composed of glycerol, fatty acids, and nitrogen-containing bases, serving as the main structural components of cell membranes and intracellular membranes. They are abundant in tissues such as the brain, nerve tissue, bone marrow, and liver. Phospholipids can be divided into three types: lecithin, cephalin, and sphingomyelin. Common methods for demonstrating phospholipids include the acidic hematoxylin method and pyridine extraction method.
Phospholipid Staining Solution (Acidic Hematoxylin Method), also known as Baker’s Phospholipid Stain, uses calcium formaldehyde to fix tissues. Calcium ions prevent phospholipids from dissolving into the fixative solution. After treatment with Baker’s mordant, phospholipids bind to metal ions to form insoluble phospholipid chelates, which then stain into blue-black complexes with acidic hematoxylin. Phospholipids and sphingomyelin appear blue-black under staining.Note: This reagent is for research use only. Not for clinical diagnosis or other purposes.
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Materials to Be Prepared by User
1. Distilled water, graded ethanol, glycerin gelatin, calcium formaldehyde fixative
2. Incubator or water bath
Operating Procedures (For Reference Only)
1. Fix tissues in calcium formaldehyde fixative for 16 hours (overnight).
2. Immerse the fixed tissues in Baker’s mordant in a 60℃ incubator for 24 hours, followed by rinsing under running water for 16 hours (overnight).
3. Section the tissues using a cryostat or semiconductor refrigeration microtome at a thickness of 8 μm.
4. Wash the sections with distilled water and air-dry.
5. Re-immerse the sections in Baker’s mordant in a 60℃ incubator for 1 hour, rinse briefly under running water, and wash again with distilled water.
6. Stain the sections in acidic hematoxylin stain in a 37℃ incubator for 2-5 hours, then rinse under running water.
7. Differentiate the sections in Baker’s differentiating solution in a 37℃ incubator for 0.5-2 hours, rinse under running water for 10 minutes, and wash with distilled water.
8. Mount the sections with glycerin gelatin.
Staining Results
Phospholipids, Sphingomyelin, Nucleoprotein: Blue–Black
Cytoplasm Grayish: Yellow
Negative Control (Optional)
1. Prepare a phospholipid extraction reagent: chloroform:methanol (2:1) or pyridine.
2. Treat consecutive tissue sections with the above extraction reagent at room temperature for 1–2 hours.
3. Perform phospholipid staining using the same procedure; the result should be negative.
Precautions
1. This staining method may produce false positives. If necessary, treat sections with a lipid extraction reagent and perform a negative control.
2. Acidic hematoxylin stain is for single use only. Do not reuse it.
3. The differentiating solution can be reused until its yellow color fades significantly, then discard it.
4. For your safety and health, wear a lab coat and disposable gloves during operation.
5. Use the reagent as soon as possible after opening to avoid affecting subsequent experimental results.
规格
别名
磷脂染色液 | Baker磷脂染色液
英文别名
Phospholipid Staining Solution
规格或纯度
BioReagent, 生物染色剂, 用于显微镜
英文名称
Phospholipid staining solution (acid hematoxylin method)
应用
Cell Staining
敏感性
Light-sensitive
储存与运输
储存条件
避光,室温
运输条件
常规运输
稳定性与储存
各组分在相应的储存条件下保质期1年
组分与储存
| P1508519 | Component | 3×100 mL | Storage |
| P1508519A | Baker媒染液 | 100 mL | RT |
| P1508519B | 酸性苏木红染色液 | 100 mL | RT. Store in the dark. |
| P1508519C | Baker分化液 | 100 mL | RT |
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
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通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0332879 |
分析证书 | P1508519 |
技术文档和文章
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