计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
原虫为单细胞真核动物，体积微小而能独立完成生命活动的全部生理功能。在自然界分布广泛，种类繁多。迄今已发现约6.5万余种，其中多数营自生或腐生生活，分布在海洋、土壤、水体或腐败物内，约有近万种为寄生性原虫，生活在动物体内或体表，另外还有少部分医疗原虫。
粪便检查是诊断寄生虫病常用的病原学检测方法。常用方法有直接涂片法、厚涂片透明法等。原虫包囊染色液 (4%碘液) 是直接涂片法的一种，是碘和碘化钾的水溶液，与Lugol's碘液相似，多用于粪便检查蠕虫卵、原虫的包囊和滋养体；其中碘的有效含量为4%，操作简单，可连续多天采样检查，提高检出率，但结果阴性不完全排除有寄生虫感染。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
操作步骤(仅供参考)：
1、根据实验具体要求操作或参考如下步骤。
2、滴1滴生理盐水与载玻片左半侧的中央，置1滴碘液与玻片右半侧的中央。
3、用小木棍或火柴杆挑起粪便约2mg(火柴头大小)，加入生理盐水滴中，并加入相似量粪便到碘液滴中。混合粪便与液滴以形成悬液。
4、用盖玻片盖住液滴，操作时应首先盖好玻片，使之与玻片成一角度，然后接触液滴边缘，并轻轻放下盖玻片到玻片上，以避免气泡产生。
5、低倍镜检查，如需鉴定，在高倍镜下，以上下或横向移动方式检查，使全部盖玻片范围都能被检查到；当见到生物体或可疑物时，调至高倍镜以观察其细微的状态。
染色结果：
蠕虫卵、原虫的包囊和滋养体呈棕色或黑色。
注意事项：
1、要取得准确的结果，粪便必须新鲜，送检时间一般不宜超过24h。如检查肠内原虫滋养体，最好立即检查，或暂时保存在35~37°C条件下待查。
2、盛粪便的容器须洁净、干燥，并防止污染；粪便不可混入尿液及其他体液等，以免影响检查结果。
3、涂片之前应事先在背面做好圆圈标记，以便判断后续试验的位置。
4、涂片过厚易致粪渣遮住虫体，过薄易存在空白区域。
5、应注意虫卵与粪便中的异物鉴别。
6、气温越接近体温，滋养体的活动越明显，必要时可略加温，一般在15min检查完毕。
7、为了您的安全和健康，请穿实验服并戴一次性手套操作。
Protozoa are unicellular eukaryotic organisms that, despite their minute size, are capable of independently performing all physiological functions required for life. They are widely distributed in nature with a great diversity of species. To date, more than 65,000 species have been identified. The majority of these are free-living or saprophytic, inhabiting oceans, soil, water bodies, or decaying matter. Approximately 10,000 species are parasitic protozoa that live inside or on the surface of animal bodies, while a small number are medically relevant protozoa.
Fecal examination is a common etiological testing method for diagnosing parasitic diseases. Commonly used techniques include the direct smear method and the thick smear clearing method. Protozoa Encystment Staining Solution (4% Iodine Solution) is a reagent designed for the direct smear method. It is an aqueous solution of iodine and potassium iodide, similar to Lugol's iodine solution. It is mostly used for detecting helminth eggs, protozoan cysts, and trophozoites in fecal samples. The effective iodine concentration of this solution is 4%. It features simple operation and allows for consecutive sampling and testing over multiple days to improve the detection rate. However, a negative result does not completely rule out parasitic infection. This reagent is intended for research use only and is not suitable for clinical diagnosis or any other purposes.
Operating Procedures (For Reference Only):
1. Perform the operation according to the specific experimental requirements or follow the steps below.
2. Place 1 drop of physiological saline in the center of the left half of a glass slide, and 1 drop of iodine solution in the center of the right half of the slide.
3. Use a wooden splint or matchstick to pick approximately 2 mg of feces (the size of a match head), add it to the drop of physiological saline, and add a similar amount of feces to the drop of iodine solution. Mix the feces with the liquid drops to form suspensions.
4. Cover the liquid drops with cover slips. To perform this step correctly, first hold the cover slip at an angle relative to the glass slide, then touch the edge of the liquid drop, and gently lower the cover slip onto the slide to avoid air bubbles.
5. Examine the slides under the low-power objective lens. If further identification is required, switch to the high-power objective lens and scan the entire area of the cover slip by moving the slide up, down, left, or right. When organisms or suspicious structures are observed, switch to the high-power objective lens to examine their detailed morphology.
Staining Results:
Helminth eggs, protozoan cysts, and trophozoites appear brown or black.
Precautons:
1. For accurate results, the fecal samples must be fresh, and the time from sample collection to submission for testing should generally not exceed 24 hours. If intestinal protozoan trophozoites are to be examined, it is best to process the samples immediately, or store them temporarily at 35–37°C prior to testing.
2. The containers used for holding fecal samples must be clean and dry to prevent contamination. Urine and other body fluids must not be mixed with the fecal samples, as this may interfere with the test results.
3. Prior to preparing the smears, mark a circle on the back of the glass slides to identify the target area for subsequent examinations.
4. Overly thick smears may cause fecal debris to obscure the parasites, while excessively thin smears tend to have blank areas without sufficient sample coverage.
5. Care should be taken to distinguish helminth eggs from foreign materials present in the feces.
6. The activity of trophozoites becomes more pronounced when the temperature is close to the body temperature. If necessary, the samples can be slightly warmed, and the entire examination should generally be completed within 15 minutes.
7. For your safety and health, wear a lab coat and disposable gloves during the operation.
规格
别名
原虫包囊碘液染色液 | 包囊碘染液
英文别名
Protozoan Cyst Iodine Stain
规格或纯度
4%碘液
稳定性与储存
Store at room temperature long term (12 months). Store in the dark.
英文名称
Protozoa Encystment Staining Solution (4% Iodine Solution)
储存条件
避光,室温
运输条件
常规运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0332386 |
分析证书 | P1508790 |
技术文档和文章
溶液计算器
常见问题
本产品应如何储存？
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