计算溶液所需的质量、体积或浓度。
植物过氧化氢酶 (CAT)提取试剂
有货
为什么选择此级别
BioReagent,植物样品抽提用 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
过氧化氢酶 (Catalase, CAT)又称触酶，是一类以铁卟啉为辅基的结合酶，由四个相同亚单位组成的四聚体酶，共含4分子的亚铁血红素作为辅基，分子量约为24KD，CAT能将细胞代谢产生的毒性物质过氧化氢迅速清除，可与GSH-Px共同保护巯基酶、膜蛋白、过氧化氢解离。
植物过氧化氢酶 (CAT)提取试剂主要用于裂解植物组织，提取植物样品中的过氧化物酶。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
自备材料：
1、蒸馏水
2、离心管或试管、匀浆器或研钵、低温离心机
操作步骤 (仅供参考)：
1、取正常或逆境下的新鲜植物组织 (或动物组织)，清洗干净，擦干，切碎，迅速称取。
2、按植物组织：植物过氧化氢酶 (CAT)提取试剂=0.5g: 2ml的比例，加入预冷的植物过氧化氢酶 (CAT)提取试剂，冰浴情况下充分捣碎或研磨。
3、转移至15ml离心管，用植物过氧化氢酶 (CAT)提取试剂冲洗研钵或匀浆器，合并冲洗液至该离心管，补加植物过氧化氢酶 (CAT)提取试剂至10ml。
4、混匀，4℃冰箱中静置10min，转移离心管上部的清液至新的离心管。
5、4℃ 1200g离心30min，留取上清液，上清液即为过氧化氢酶粗提液。
6、4℃保存备用，用于CAT的检测或其他用途。
计算：
样品粗酶液获得率 (ml/g)=上清液体积 (ml)/样品质量 (g)×100%
注意事项：
1、待测样品中不能含有磷酸酶抑制剂，同时需避免反复冻融。
2、所测样本的值高于标准曲线的上限，应用提取试剂稀释样品后重新测定。
3、试剂开封后请尽快使用，以防影响后续实验效果。
4、为了您的安全和健康，请穿实验服并戴一次性手套操作。
Catalase (CAT), also known as contact enzyme, is a type of conjugate enzyme with iron porphyrin as the prosthetic group. It is a tetrameric enzyme composed of four identical subunits, containing a total of 4 molecules of ferrous heme as prosthetic groups, with a molecular weight of approximately 24 KD. CAT can rapidly scavenge hydrogen peroxide, a toxic substance produced by cellular metabolism, and work together with GSH-Px to protect sulfhydryl enzymes, membrane proteins and dissociate hydrogen peroxide.
The Plant Catalase (CAT) Extraction Reagent is primarily designed for lysing plant tissues and extracting catalase from plant samples. This reagent is intended for research use only and is not suitable for clinical diagnosis or any other applications.
Materials to Be Prepared by the User
1. Distilled water
2. Centrifuge tubes or test tubes, homogenizer or mortar and pestle, refrigerated centrifuge
Operating Procedures (For Reference Only)
1. Take fresh plant tissues (or animal tissues) under normal or stress conditions, rinse them thoroughly, blot them dry, cut them into small pieces, and weigh them quickly.
2. Add pre-cooled Plant Catalase (CAT) Extraction Reagent at a ratio of 0.5 g plant tissue: 2 ml reagent, then fully homogenize or grind the mixture under ice bath conditions.
3. Transfer the homogenate into a 15 ml centrifuge tube, rinse the mortar or homogenizer with Plant Catalase (CAT) Extraction Reagent, combine the rinsing solution into the same centrifuge tube, and make up the volume to 10 ml with the reagent.
4. Mix well, let it stand in a 4°C refrigerator for 10 minutes, then transfer the upper clear solution into a new centrifuge tube.
5. Centrifuge at 1,200×g and 4°C for 30 minutes, collect the supernatant, which is the crude catalase extract.
6. Store the supernatant at 4°C for later use in CAT activity assays or other experimental applications.
Calculation
Yield of crude enzyme solution from samples (ml/g)=Volume of supernatant (ml)/Mass of sample (g)×100%
Precautions
1. The sample to be tested must not contain phosphatase inhibitors, and repeated freeze-thaw cycles should be avoided.
2. If the measured value of the sample exceeds the upper limit of the standard curve, dilute the sample with the extraction reagent and perform the assay again.
3. Please use the reagent as soon as possible after opening to prevent it from affecting the results of subsequent experiments.
4. For your safety and health, wear a lab coat and disposable gloves during the operation.
规格
别名
植物过氧化氢酶提取试剂 | CAT提取试剂
英文别名
Plant Catalase Extraction Reagent | CAT Extraction Reagent
规格或纯度
BioReagent, 植物样品抽提用
稳定性与储存
Store at 2-8℃ long term (12 months).
英文名称
Plant Catalase (CAT) Extraction Reagent
储存条件
2-8°C储存
运输条件
低温运输
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0232127 |
分析证书 | P1509586 |
技术文档和文章
溶液计算器
选择需要帮助选择级别？
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Suitable for plant cell and tissue extracts 级别指南 →产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 保留所有权利。
危险品化学品经营许可证（带存储）