天然果胶类物质以原果胶、果胶 (Pectin)、果胶酸的形态广泛存在于植物的果实、根、茎、叶中,是细胞壁的一种组成成分,它们伴随纤维素而存在,构成相邻细胞中间层粘结物,使植物组织细胞紧紧黏结在一起。果胶酶 (Pectinase)是一类分解果胶质酶类的总称,实质是多聚半乳糖醛酸水解酶,包括原果胶酶,果胶酯酶,多聚半乳糖醛酸酶和果胶裂解酶四大类,广泛存在于植物果实和微生物中,主要用于食品、酿酒、环保、医药、纺织及日化用品行业。
果胶酶提取试剂主要用于提取植物组织或果实中的果胶酶,500ml可提取160次样本左右。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
自备材料:
1、蒸馏水
2、实验材料:桃子、李子、苹果、杏等果实或其他植物组织
3、电子天平、水果刀或剪刀、研钵或匀浆器、试管或离心管、离心机
操作步骤 (仅供参考):
1、取果实或其他植物组织,洗净,擦干,称取剪碎的新鲜样品3g,置于提前4℃预冷的研钵或匀浆器。
2、加入3ml 4℃预冷的果胶酶提取试剂,充分研磨或匀浆后转入离心管或试管。
3、4℃ 10000g离心10min,留取上清液,即为果胶酶粗提液。
注意事项:
1、果胶酶提取试剂应密闭保存,避免有效成分挥发。
2、试剂开封后请尽快使用,以防影响后续实验效果。
3、为了您的安全和健康,请穿实验服并戴一次性手套操作。
Natural pectin substances exist widely in fruits, roots, stems and leaves of plants in the forms of protopectin, pectin and pectic acid. They are components of plant cell walls, existing together with cellulose and forming the intercellular cement between adjacent cells, which tightly binds plant tissue cells together. Pectinase is a general term for a class of enzymes that decompose pectin substances, essentially polygalacturonase hydrolase. It includes four major categories: protopectinase, pectinesterase, polygalacturonase and pectin lyase. Pectinase is widely found in plant fruits and microorganisms, and is mainly used in food, wine-making, environmental protection, medicine, textile and daily chemical industries.
This Pectinase Extraction Reagent is mainly used for extracting pectinase from plant tissues or fruits. 500 ml of the reagent can be used for approximately 160 sample extractions. Note: This reagent is intended for research purposes only and not for clinical diagnosis or any other applications.
Materials to Be Prepared by the User
1. Distilled water
2. Experimental materials: Fruits such as peaches, plums, apples and apricots, or other plant tissues
3. Electronic balance, fruit knife or scissors, mortar or homogenizer, test tubes or centrifuge tubes, centrifuge
Operating Procedures (For Reference Only)
1. Take the fruits or other plant tissues, rinse them thoroughly, dry them, weigh 3 g of the cut fresh samples, and place them in a mortar or homogenizer pre-cooled at 4℃ in advance.
2. Add 3 ml of the Pectinase Extraction Reagent pre-cooled at 4℃, grind or homogenize the mixture fully, and then transfer it into a centrifuge tube or test tube.
3. Centrifuge the mixture at 10000 g for 10 min at 4℃, and collect the supernatant, which is the crude pectinase extract.
Precautions
1. The Pectinase Extraction Reagent should be stored in a sealed container to avoid volatilization of active ingredients.
2. Please use the reagent as soon as possible after opening to prevent affecting the subsequent experimental results.
3. For your safety and health, wear a lab coat and disposable gloves during operation.