氨基酸 (Amino acid, AA)是组成蛋白质的基本单位,也是蛋白质的分解产物,动物肝脏、肾脏是氨基酸代谢的主要器官,故尿中氨基酸的变化最能反应肝、肾的生理状态,植物体内氨基酸含量对研究植物在不同条件下及不同生长发育时期氮代谢变化、植物对氮素的吸收、运输、同化及营养状况等有重要意义,另外氨基酸还能反映灼伤、伤寒等方面情况。
植物氨基酸 (AA)提取试剂主要用于裂解植物组织,提取植物组织、食品、药品等中的总游离氨基酸含量。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
自备材料:
1、去离子水或无氨蒸馏水
2、离心管或试管、匀浆器或研钵
操作步骤 (仅供参考):
1、植物样品:取新鲜植物组织,清洗干净,擦干,切碎,迅速称取0.5g。
2、按植物组织: 植物氨基酸提取试剂=1g: 5ml的比例加氨基酸提取试剂,匀浆或研磨。
3、用去离子水稀释至10ml,混匀,用滤纸过滤,滤液即为氨基酸粗提液,4℃保存备用。
4、如果样品中含有较高浓度的氨基酸,可以使用植物氨基酸提取试剂进行恰当的稀释。
注意事项:
1、实验材料应尽量新鲜,如取材后不立即使用,应存于4℃。
2、植物氨基酸提取试剂应密闭保存,避免挥发。
3、所测样本的浓度过高,应用植物氨基酸提取试剂稀释样品。
4、试剂开封后请尽快使用,以防影响后续实验效果。
5、为了您的安全和健康,请穿实验服并戴一次性手套操作。
Amino acids (AA) are the basic units of proteins and also the degradation products of proteins. The liver and kidneys of animals are the primary organs for amino acid metabolism. Therefore, changes in amino acids in urine can best reflect the physiological status of the liver and kidneys. The amino acid content in plants is of great significance for studying nitrogen metabolism changes in plants under different conditions and at different growth and development stages, as well as plant nitrogen uptake, transport, assimilation, and nutritional status. In addition, amino acids can also indicate conditions such as burns and typhoid fever.
This Plant Amino Acid (AA) Extraction Reagent is mainly used for lysing plant tissues and extracting the total free amino acid content from plant tissues, food, pharmaceuticals, and other samples. Note: This reagent is intended for research purposes only and not for clinical diagnosis or any other applications.
Materials to Be Prepared by the User
1. Deionized water or ammonia-free distilled water
2. Centrifuge tubes or test tubes, homogenizers or mortars
Operating Procedures (For Reference Only)
1. Plant Samples: Take fresh plant tissues, rinse them thoroughly, dry them, cut them into small pieces, and weigh 0.5 g of the samples quickly.
2. Add the amino acid extraction reagent at a ratio of 1 g plant tissue: 5 ml extraction reagent, then homogenize or grind the mixture.
3. Dilute the homogenate to 10 ml with deionized water, mix well, and filter the solution with filter paper. The filtrate is the crude amino acid extract, which can be stored at 4℃ for later use.
4. If the sample contains a high concentration of amino acids, it can be appropriately diluted with the Plant Amino Acid Extraction Reagent.
Precautions
1. Experimental materials should be as fresh as possible. If not used immediately after sampling, they should be stored at 4℃.
2. The Plant Amino Acid Extraction Reagent should be stored in a sealed container to avoid volatilization.
3. If the concentration of the measured sample is too high, dilute the sample with the Plant Amino Acid Extraction Reagent.
4. Please use the reagent as soon as possible after opening to prevent affecting the subsequent experimental results.
5. For your safety and health, wear a lab coat and disposable gloves during operation.