平衡盐溶液 (Balanced Salt Solution,BSS)与细胞生长状态下的pH值、渗透压等环境状态一致,具有维持渗透压、控制酸碱平衡、供给细胞生存代谢所必需的能量和无机盐成分等作用,可满足体外实验中细胞生存并维持一定代谢的基本需要,主要由无机离子组成,有时含有碳酸氢钠、葡萄糖、酚红等,如果有必要还可以加入HEPES以维持渗透压的平衡。平衡盐溶液可以作为完全培养基的基液,亦可以用于稀释浓缩的氨基酸、维生素溶液以配制完全培养基。BSS配方常有改动,如Hank's BSS有不含钙镁或酚红的,也有含钙镁含酚红的等等,Dulbecco's PBS有不含钙镁或含钙镁的等,HBSS、EBSS、PBS等都是与较弱的磷酸盐缓冲液相关的盐溶液,常见的平衡盐溶液有Eargle's液、Hank's液、磷酸盐缓冲溶液 (PBS)等。
PBS-EDTA缓冲液 (PE, 10 mmol/L)主要由PBS和10 mmol/L EDTA等组成,pH值约为7.4,可用于细胞缓冲液、螯合金属离子、培养细胞的消化,该试剂经高压灭菌。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
操作步骤 (仅供参考):
1、根据实验具体要求操作。
2、大多数情况下PE的使用浓度为1 mmol/L,即使用时用PBS稀释10倍;当需要螯合金属离子 (尤其是Ca²⁺、Mg²⁺)时,浓度应提高到5 mmol/L,即稀释1倍。
注意事项:
1、注意无菌操作,避免被微生物污染。
2、该试剂经高压灭菌处理。试剂开封后请尽快使用,以防影响后续实验效果。
3、为了您的安全和健康,请穿实验服并戴一次性手套操作。
Balanced Salt Solution (BSS) matches the environmental conditions such as pH value and osmotic pressure required for cell growth. It functions to maintain osmotic pressure, regulate acid-base balance, and supply energy and inorganic salt components essential for cell survival and metabolism. BSS can meet the basic needs of cell survival and sustained metabolism in in vitro experiments. It is mainly composed of inorganic ions, and sometimes contains sodium bicarbonate, glucose, phenol red, etc. If necessary, HEPES can also be added to maintain osmotic pressure balance. Balanced Salt Solution can be used as the base solution of complete medium, or for diluting concentrated amino acid and vitamin solutions to prepare complete medium. The formulation of BSS is often modified. For example, Hank's BSS is available in variants without calcium, magnesium or phenol red, as well as those containing calcium, magnesium and phenol red; Dulbecco's PBS also has versions with or without calcium and magnesium. HBSS, EBSS, PBS and others are all salt solutions related to weakly buffered phosphate solutions. Common balanced salt solutions include Earle's solution, Hank's solution, Phosphate-Buffered Saline (PBS) and so on.
PBS-EDTA Buffer (PE, 10 mmol/L) is mainly composed of PBS and 10 mmol/L EDTA, with a pH value of approximately 7.4. It can be used as a cell buffer, for chelating metal ions, and for the digestion of cultured cells. This reagent has undergone autoclaving sterilization. This reagent is for research use only and not intended for clinical diagnosis or any other purposes.
Operating Procedures (for reference only)
1. Operate according to the specific requirements of the experiment.
2. In most cases, the working concentration of PE is 1 mmol/L, i.e., dilute it 10-fold with PBS before use; when it is necessary to chelate metal ions (especially Ca²⁺, Mg²⁺), the concentration should be increased to 5 mmol/L, i.e., dilute it 2-fold.
Precautions
1. Ensure aseptic operation to avoid microbial contamination.
2. This reagent has been autoclaved. Please use the reagent as soon as possible after opening to prevent affecting the results of subsequent experiments.
3. For your safety and health, wear a lab coat and disposable gloves during operation.