计算溶液所需的质量、体积或浓度。
植物组织染色液（Delafield苏木素-番红法）
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
植物组织染色方法多样，操作流程与动物组织染色相近。Delafield 苏木素（代氏苏木素）是适用于非木质化组织的优良染色剂，与番红搭配进行对染，对多种植物病害组织可获得理想的染色效果。
植物组织染色液（Delafield苏木素-番红法）主要由 Delafield 苏木素染色液、番红染色液等组分组成，可将木质化组织染为红色，菌丝等结构染为蓝色。本品仅用于科研领域，不适用于临床诊断及其他非科研用途。
自备材料
10%中性福尔马林固定液、蒸馏水、系列乙醇
操作步骤（仅供参考）
1. 标本的处理：固定，切成石蜡切片。
2. 粘片：材料切成薄片，将切片粘在载玻片上，加热展开，所用载玻片必须清洁；先把粘贴剂置于载玻片上，再取切片，浮置胶液上，然后置烘片台上，使切片烫平，以材料不出现皱纹为宜。
3. 脱蜡：二甲苯→二甲苯+无水乙醇 (1+1) →100%乙醇→95%乙醇→85%乙醇→70%乙醇→50%乙醇→30%乙醇→水，以上各级需要5～10 min。
4. 入Delafield苏木素染色液浸染30 min，水洗1 min。
5. 酸性分化液分色，自来水冲洗。
6. 入30%乙醇1 min，再入50%乙醇1 min。
7. 入番红染色液染色1～6 h，对于特殊组织可延长至12 h。
8. 脱色：50%乙醇→70%乙醇→80%乙醇→90%乙醇→95%乙醇，以上各级需要1～2 min。
9. 50%二甲苯+50%乙醇透明2 min，二甲苯2 min，二甲苯10 min。
10. 树脂封固，及时镜检。
染色结果
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注意事项
1. 番红染色后在50%乙醇中脱色需经实践，如果脱色不够绿色会不好染；脱色过度会导致红色过淡。
2. 二甲苯可以用脱蜡透明液替代，该产品为环保材料。
3. 染色时间不是绝对的，常因材料种类、切片厚度不同而不同。
4. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
5. 试剂开封后请尽快使用，以防影响后续实验效果。
Plant tissue staining methods are diverse, and their operational procedures are similar to those used for animal tissue staining. Delafield’s hematoxylin is an excellent stain suitable for non-lignified tissues. When used in combination with safranin as a counterstain, it can achieve ideal staining results for various plant disease tissues.
The Plant Tissue Staining Solution (Delafield’s Hematoxylin-Safranin Method) mainly consists of Delafield’s Hematoxylin Staining Solution, Safranin Staining Solution, and other components. It stains lignified tissues red and structures such as fungal hyphae blue. This product is intended for research use only and is not suitable for clinical diagnosis or other non-research purposes.
Materials to Be Prepared by User
10% neutral formalin fixative, distilled water, graded ethanol series
Procedure (For Reference Only)
Sample Processing: Fix the specimen and prepare paraffin sections.
Section Mounting: Cut the material into thin sections. Mount the sections onto clean glass slides and heat them to flatten. First, apply an adhesive to the slide, then place the section onto the adhesive solution. Position the slide on a warming plate to flatten the section, ensuring no wrinkles appear.
Dewaxing: Xylene → Xylene + absolute ethanol (1:1) → 100% ethanol → 95% ethanol → 85% ethanol → 70% ethanol → 50% ethanol → 30% ethanol → water. Incubate for 5–10 minutes at each step.
Stain in Delafield’s Hematoxylin Staining Solution for 30 minutes, then rinse with water for 1 minute.
Differentiate with Acidic Differentiation Solution and rinse with tap water.
Transfer to 30% ethanol for 1 minute, then to 50% ethanol for 1 minute.
Stain with Safranin Staining Solution for 1–6 hours. For special tissues, staining can be extended up to 12 hours.
Destaining: 50% ethanol → 70% ethanol → 80% ethanol → 90% ethanol → 95% ethanol. Incubate for 1–2 minutes at each step.
Clearing: 50% xylene + 50% ethanol for 2 minutes → xylene for 2 minutes → xylene for 10 minutes.
Mount with resin and examine under a microscope promptly.
Staining Results
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Notes
Destaining in 50% ethanol after safranin staining requires practice. Insufficient destaining may affect subsequent staining, while excessive destaining can result in overly faint red coloration.
Xylene can be replaced with a dewaxing/clearing solution, which is an environmentally friendly alternative.
Staining times are not absolute and often vary depending on the type of material and section thickness.
For your safety and health, please wear a lab coat and disposable gloves during operation.
Use the reagents as soon as possible after opening to avoid affecting subsequent experimental results.
规格
别名
Delafield苏木素-番红植物组织染色液 | 植物组织苏木素番红双重染色液 | 番红‑Delafield 苏木素染色液 | 植物切片染色液（Delafield 苏木素‑番红法） | 植物组织常规染色液（苏木素‑番红法）
英文别名
Delafield Hematoxylin and Safranin Stain for Plant Tissues | Safranin‑Delafield Hematoxylin Double Stain | Plant Histology Stain (Delafield Hematoxylin‑Safranin) | Delafield‑Safranin Staining Solution for Plant Sections | Plant Tissue Dual Stain (Hematoxy
规格或纯度
BioReagent,生物染色剂,用于显微镜
英文名称
Plant Tissue Staining Solution (Delafield Hematoxylin‑Safranin Method)
储存与运输
储存条件
室温
运输条件
常规运输
稳定性与储存
Store at room temperature long term (12 months).
组分与储存
| P1511516 | Component | 3×50 mL | Storage |
| P1511516A | Delafield苏木素染色液 | 50 mL | RT. |
| P1511516B | 酸性分化液 | 100 mL | RT. |
| P1511516C | 番红染色液 | 50 mL | RT. |
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0434172 |
分析证书 | P1511516 |
技术文档和文章
溶液计算器
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