计算溶液所需的质量、体积或浓度。
植物胼胝质染色液（苯胺蓝荧光法）
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜,适用于荧光分析 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
胼胝质（callose）是一类由葡萄糖残基以不同于纤维素的方式连接形成的非纤维素多糖，在植物筛管代谢、配子体发育等生命活动中发挥重要调控作用，其合成与降解直接影响植物正常生长代谢，是植物研究的重要内容。
植物胼胝质染色液（苯胺蓝荧光法）依据苯胺蓝与植物胼胝质特异性结合、并在特定波长下激发出黄绿色荧光的原理制备，操作简便快捷。本品仅用于科研领域，不可用于临床诊断及其他非科研用途。
自备材料
- AAF固定液或Carnoy固定液、50%乙醇、无水乙醇、1×PBS、甘油明胶封片剂或抗荧光淬灭封片剂
- 新鲜的植物组织
操作步骤（仅供参考）
1. 配制苯胺蓝染色工作液：将苯胺蓝染色液和苯胺蓝稀释液等比例混合，现配现用。
2. 新鲜植物组织切成2 mm左右的薄片，植物叶片推荐裁剪成1 cm×2 cm，幼嫩植物叶片可直接浸于AAF固定液或Carnoy固定液固定24小时。
3. 使用无水乙醇浸洗两次，每次1分钟，然后转入至少10倍体积的100%乙醇中保存。
4. 染色前取出组织，浸于50%乙醇中平衡30 min，取出稍沥干。
5. 随后浸于1×PBS中平衡30 min，取出稍沥干。
6. 组织切片滴加或浸于苯胺蓝染色工作液中，室温避光染色1~2小时。
7. 于载玻片上滴加25 μL水性甘油明胶封片剂或抗荧光淬灭封片剂，小心的将染色后的组织转移至载玻片上，继续滴加少量封片剂封片。
8. 在荧光显微镜下观察，紫外光激发，激发波长为330~385 nm (BP)，发射波长为425 nm (LP)。用未经染色的切片做对照。
染色结果
胼胝质（紫外光下）呈黄绿色荧光，背景无着色。
注意事项
1. 在条件允许下，推荐将浸于固定液中的植物组织负压真空处理20 min，有助于固定液的渗透。
2. 如暂时不进行实验或需同时处理大量样本，浸于100%乙醇的样本可于2-8℃保存至少1周。
3. 样本染色完成后尽快拍照保存结果。
4. 染色液有效成分易分解，建议临用前配置，在 3 小时内使用。使用过程中可能出现试剂颜色少量变浅，属于正常现象。
5. 荧光染色容易发生光淬灭，在染色和转移以及观察过程中注意避免激发光以外的环境光。
6. 试剂开封后请尽快使用，以防影响后续实验效果。
7. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
Callose is a type of non-cellulosic polysaccharide composed of glucose residues linked in a manner distinct from cellulose. It plays a crucial regulatory role in key biological processes such as plant sieve tube metabolism and gametophyte development. The processes of its synthesis and degradation directly impact normal plant growth and metabolism, making it a significant subject in plant research.
Plant Callose Staining Solution (Aniline Blue Fluorescence Method) is prepared based on the principle of specific binding between aniline blue and plant callose, which emits a yellow-green fluorescence under specific wavelengths. This method is simple and fast to operate. This product is for research use only and is not suitable for clinical diagnosis or other non-research purposes.
Materials to Be Prepared by User
- AAF Fixative Solution or Carnoy's Fixative Solution, 50% Ethanol, Absolute Ethanol, 1× PBS, Glycerol-Gelatin Mounting Medium or Anti-Fade Mounting Medium
- Fresh plant tissue
Procedure (For Reference Only)
Prepare the Aniline Blue Working Solution: Mix the Aniline Blue Staining Solution and Aniline Blue Diluent in equal proportions. Prepare immediately before use.
Cut fresh plant tissue into slices approximately 2 mm thick. For plant leaves, it is recommended to cut them into pieces about 1 cm × 2 cm. Tender plant leaves can be directly immersed in AAF Fixative Solution or Carnoy's Fixative Solution for 24 hours.
Rinse the tissue twice with absolute ethanol, 1 minute each time. Then transfer and store the tissue in at least 10 times its volume of 100% ethanol.
Before staining, retrieve the tissue and immerse it in 50% ethanol for equilibration for 30 minutes. Remove and allow to drain slightly.
Subsequently, immerse the tissue in 1× PBS for equilibration for 30 minutes. Remove and allow to drain slightly.
Apply the Aniline Blue Working Solution dropwise onto the tissue section or immerse the section in the working solution. Stain at room temperature, protected from light, for 1~2 hours.
Place 25 μL of aqueous Glycerol-Gelatin Mounting Medium or Anti-Fade Mounting Medium on a glass slide. Carefully transfer the stained tissue onto the slide, and add a small additional amount of mounting medium before coverslipping.
Observe under a fluorescence microscope using UV excitation light (excitation wavelength 330~385 nm (BP), emission wavelength 425 nm (LP)). Use an unstained section as a control.
Staining Results
Callose appears as yellow-green fluorescence under UV light, with no background staining.
Notes
When conditions permit, it is recommended to subject the plant tissue immersed in fixative to negative pressure/vacuum treatment for 20 minutes to aid fixative penetration.
If the experiment is not to be performed immediately or a large number of samples need to be processed simultaneously, samples stored in 100% ethanol can be kept at 2-8°C for at least one week.
Photograph the stained samples as soon as possible after staining to preserve the results.
The active components of the staining solution are prone to decomposition. It is recommended to prepare the working solution immediately before use and use it within 3 hours. A slight color fading of the reagent during use is a normal phenomenon.
Fluorescent staining is susceptible to photobleaching. Take care to avoid ambient light other than the excitation light during staining, transfer, and observation.
Use the reagents as soon as possible after opening to avoid affecting subsequent experimental results.
For your safety and health, please wear a lab coat and disposable gloves during operation.
规格
别名
苯胺蓝荧光胼胝质染色液 | 水溶性苯胺蓝胼胝质荧光染色液 | 胼胝质荧光染色液（苯胺蓝法） | 植物胼胝质苯胺蓝荧光染色试剂 | 胼胝质特异荧光染色液（苯胺蓝染色法）
英文别名
Aniline Blue Fluorescent Stain for Plant Callose | Water-Soluble Aniline Blue Callose Fluorescent Staining Solution | Callose Fluorescence Staining Solution (Aniline Blue) | Aniline Blue Fluorescence Assay Solution for Plant Callose | Plant Callose Detect
规格或纯度
BioReagent,生物染色剂,用于显微镜,适用于荧光分析
英文名称
Plant Callose Staining Solution (Aniline Blue Fluorescence Method)
敏感性
Light-sensitive
Emission(nm)
425nm
Excitation(nm)
330-385nm
Ex/Em(nm)
330-385/425nm
储存与运输
储存条件
避光,室温
运输条件
常规运输
稳定性与储存
Store at room temperature long term (12 months). Store in the dark.
组分与储存
| P1511523 | Component | 2×25 mL | Storage |
| P1511523A | 苯胺蓝染色液 | 25 mL | RT. Store in the dark. |
| P1511523B | 苯胺蓝稀释液 | 25 mL | RT. Store in the dark. |
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0434175 |
分析证书 | P1511523 |
技术文档和文章
溶液计算器
常见问题
什么是「生物染色剂」？
「生物染色剂」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
什么是「BioReagent」？
「BioReagent」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
本产品应如何储存？
请于室温、避光条件下保存。本品对光敏感，请保存在原始的避光或棕色容器中。
本产品如何运输？
本产品按常温标准条件运输，无需温控包装。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
需要帮助选择级别？
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Biological Stain 级别指南 → 查看 for Fluorescence analysis 级别指南 → 查看 for Microscopy 级别指南 →产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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