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多酚氧化酶(polyphenol oxidase，PPO)是自然界中分布极广的一种金属蛋白酶，普遍存在于植物、真菌、昆虫的质体中，甚至在土壤中腐烂的植物残渣上都可以检测到多酚氧化酶的活性，多酚氧化酶是一种蛋白体，在茶树生命活动和茶叶加工过程中参与一系列由酶促活动而引起的化学变化，故又被称为生物催化剂，该酶属于细胞木质素合成途径中间的关键酶，研究该酶可以探讨多种生物细胞发育过程中木质素沉积的代谢机理，为减少水果石细胞含量提高其品质提供依据。 该试剂盒检测原理是PPO能催化分子态氧将酚类化合物(一元酚或二元酚)氧化为醌类，常以邻苯二酚作为底物，其氧化产物是茶褐色化合物邻苯二醌，在410~450nm区间内有特异吸收峰，可通过分析邻苯醌，检测PPO的酶活性大小。本产品在酶促反应的最适条件下采用每隔一定时间测定产物生成量的方法，于分光光度计420nm处检测吸光度，以吸光度变化所需酶量进行计算。 主要用于植物组织的裂解液或匀浆液、血清等样品中内源性的多酚氧化酶活性，尤其适用于检测水果、蔬菜或茶叶中多酚氧化酶活性。仅用于科研领域，不适用于临床诊断或其他用途。
Polyphenol Oxidase (PPO) is a widely distributed metalloproteinase found extensively in nature. It is commonly present in the plastids of plants, fungi, and insects, and its activity can even be detected in decaying plant residues in soil. As a proteinaceous entity, PPO participates in a series of enzymatically driven chemical changes during the life activities of tea plants and tea processing, hence it is also referred to as a biological catalyst. This enzyme is a key enzyme in the intermediate pathway of cellular lignin biosynthesis. Studying PPO can help elucidate the metabolic mechanisms of lignin deposition during the development of various biological cells, providing a basis for reducing stone cell content in fruits and improving their quality. PPO catalyzes the oxidation of phenolic compounds (monophenols or diphenols) by molecular oxygen into quinones. Catechol is commonly used as a substrate, and its oxidation product is a tawny-colored compound, ortho-benzoquinone, which exhibits a specific absorption peak within the wavelength range of 410–450 nm. By analyzing the formation of ortho-benzoquinone, the enzymatic activity of PPO can be determined. Under optimal reaction conditions, this product measures the amount of product generated at specific time intervals. Absorbance is read at 420 nm using a spectrophotometer, and the enzyme activity is calculated based on the amount of enzyme required for the change in absorbance. This product is primarily used to measure the endogenous activity of polyphenol oxidase in samples such as lysates or homogenates of plant tissues, serum, etc. It is particularly suitable for detecting PPO activity in fruits, vegetables, or tea leaves. It is intended for research use only and is not suitable for clinical diagnosis or other purposes.
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