BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
脯氨酸(Proline，Pro)是一种环状的α-亚氨基酸，呈中性，等电点为6.30，水中溶解度比任何氨基酸都大，25℃时100g水中可溶162g左右，易潮解不易得结晶，有甜味。脯氨酸与茚三酮溶液共热，生成黄色化合物，一旦进入肽链后，可发生羟基化作用，从而形4-羟脯氨酸，是组成动物胶原蛋白的重要成分，羟脯氨酸也存在于多种植物蛋白质中，尤其与细胞壁的形成有关，在正常情况下脯氨酸含量较低，但在逆境下(旱、热、冷、冻、盐碱等)，常有脯氨酸的明显积累，即积累指数与植物的抗逆性有关，在临床、生物材料、工业等方面均有广泛应用。 该试剂盒的检测原理是脯氨酸游离于磺基水杨酸溶液中，前者与酸性茚三酮共热发生反应，产生稳定的红色产物，以酶标仪测定520nm处吸光度值，在一定浓度范围内颜色深浅与脯氨酸浓度成正比。主要用于测定植物组织中的游离脯氨酸含量，仅用于科研领域，不宜用于临床诊断或其他用途。
Proline (Pro) is a cyclic α-imino acid with a neutral nature and an isoelectric point of 6.30. It exhibits greater solubility in water than any other amino acid, with approximately 162g soluble in 100g of water at 25°C. It is hygroscopic and does not readily form crystals, possessing a sweet taste. When heated with ninhydrin solution, proline produces a yellow compound. Once incorporated into a peptide chain, it can undergo hydroxylation to form 4-hydroxyproline, a crucial component of animal collagen. Hydroxyproline is also present in various plant proteins, particularly in relation to cell wall formation. Under normal conditions, proline levels are relatively low; however, under stress conditions such as drought, heat, cold, freezing, or salinity, a significant accumulation of proline often occurs. The accumulation index is correlated with plant stress tolerance, and proline has wide applications in clinical settings, biomaterials, and industry. The detection principle of this kit is based on the reaction between free proline in a sulfosalicylic acid solution and acidic ninhydrin upon heating, which generates a stable red product. The absorbance at 520 nm is measured using a microplate reader, and within a certain concentration range, the intensity of the color is proportional to the proline concentration. This kit is primarily used for determining the free proline content in plant tissues and is intended for research purposes only. It is not suitable for clinical diagnosis or other applications.
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