PMSF(Phenylmethanesulfonyl fluoride,苯甲基磺酰氟)是一种不可逆的丝氨酸蛋白酶抑制剂,分子式为C₇H₇FO₂S,分子量174.19 g/mol。本产品为100 mM浓度的PMSF储存液,以无水乙醇为溶剂配制而成,可特异性抑制胰蛋白酶、胰凝乳蛋白酶、凝血酶等丝氨酸蛋白酶的活性,有效防止细胞或组织裂解过程中目标蛋白的降解,广泛应用于蛋白提取、纯化及后续的蛋白检测、酶切等分子生物学实验。
本产品仅用于科研领域,不适用于临床诊断、食品、化妆品等其他用途。
使用说明
本产品为100mM储存液,实验中常规工作终浓度为0.1-1mM,需用实验缓冲液(如PBS、裂解液)稀释后使用。
注意事项
1. PMSF有轻微毒性,小心操作。
2. 为了您的安全和健康,请穿实验服并戴一次性手套操作。
3. 本产品仅供科研使用,严禁它用。
PMSF (Phenylmethanesulfonyl fluoride) is an irreversible serine protease inhibitor with the molecular formula C₇H₇FO₂S and a molecular weight of 174.19 g/mol. This product is a 100 mM PMSF stock solution prepared in anhydrous ethanol. It specifically inhibits the activity of serine proteases such as trypsin, chymotrypsin, and thrombin, effectively preventing the degradation of target proteins during cell or tissue lysis. It is widely used in protein extraction, purification, and subsequent molecular biology experiments such as protein detection and enzymatic digestion.
This product is intended for research use only and is not suitable for clinical diagnosis, food, cosmetics, or other purposes.
Instructions for Use
This product is a 100 mM stock solution. The typical working concentration in experiments is 0.1–1 mM. It should be diluted with experimental buffers (e.g., PBS, lysis buffer) before use.
Precautions
1.PMSF is mildly toxic; handle with care.
2.For your safety and health, please wear laboratory clothing and disposable gloves during operation.
3.This product is for research use only and is strictly prohibited for other purposes.