PCR扩增缓冲液(10×, pH 8.3)是聚合酶链式反应(Polymerase Chain Reaction, PCR)的核心辅助试剂,为PCR反应提供稳定的酸碱环境和必要的离子条件,保障Taq DNA聚合酶等PCR相关酶的活性,促进引物与模板DNA的特异性结合,从而高效完成目的片段的扩增。
本产品的主要成分为100mM Tris-HCl缓冲液 (pH 8.3)、15mM Mg²⁺、500mM KCl等。
操作步骤(仅供参考)
1. 按实验具体要求操作。
2. PCR扩增缓冲液 (10×, pH 8.3) 在使用时工作浓度为1×。
注意事项
1. 为避免扩增效率下降,应避免反复冻融。
2. 避免微生物污染。
3. 为了您的安全和健康,请穿实验服并戴一次性手套操作。
4. 本产品仅供科研使用,严禁它用。
PCR Amplification Buffer (10×, pH 8.3) is a core auxiliary reagent for the Polymerase Chain Reaction (PCR). It provides a stable pH environment and necessary ionic conditions for the PCR reaction, ensures the activity of Taq DNA polymerase and other PCR-related enzymes, promotes the specific binding of primers to template DNA, and thereby facilitates the efficient amplification of target fragments.
The main components of this product include 100 mM Tris-HCl buffer (pH 8.3), 15 mM Mg²⁺, and 500 mM KCl, among others.
Procedure (For Reference Only)
Operate according to the specific requirements of the experiment.
PCR Amplification Buffer (10×, pH 8.3) should be used at a working concentration of 1×.
Notes
To avoid a decrease in amplification efficiency, repeated freezing and thawing should be avoided.
Prevent microbial contamination.
For your safety and health, please wear a lab coat and disposable gloves during operation.
This product is for research use only and is strictly prohibited for other purposes.