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酚氧化酶（PO，EC 1.14.18.1），也叫酪氨酸酶，是一种含有铜离子结合位点的氧化酶。在昆虫中多称为酚氧化酶。作为一类重要的免疫蛋白，在昆虫免疫防御、表皮鞣化、黑化和伤口愈合等过程中发挥重要作用。酚氧化酶（PO）能够催化L-多巴产生醌，后者在490nm有特征光吸收，通过检测490nm吸收波长的变化速率即可得出PO酶活性大小。
Phenoloxidase (PO, EC 1.14.18.1), also known as tyrosinase, is an oxidase that contains a copper-binding site. In insects, it is commonly referred to as phenoloxidase. As an important class of immune proteins, it plays a vital role in processes such as insect immune defense, cuticle sclerotization, melanization, and wound healing. Phenoloxidase (PO) catalyzes the oxidation of L-DOPA to produce quinones, which exhibit characteristic light absorption at 490 nm. By measuring the rate of change in absorbance at 490 nm, the activity of the PO enzyme can be determined.
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