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PTIO(2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide )又名2-苯基-4,4,5,5-四甲基咪唑啉-1-氧代 3-氧化物,主要用于测定生物样本和食品的抗氧化能力。 PTIO⁺·自由基离子的最大吸收波长为557nm,所以,用A₅₅₇可以检测PTIO⁺·自由基离子的浓度。如果A₅₅₇减小,表明PTIO⁺·自由基离子被清除,进而对样本中PTIO清除能力进行定量分析。
PTIO (2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide), also known as 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide, is primarily used to determine the antioxidant capacity of biological samples and food. The maximum absorption wavelength of the PTIO⁺· free radical ion is 557 nm. Therefore, the concentration of PTIO⁺· free radical ions can be detected by measuring A₅₅₇ nm. A decrease in A₅₅₇ nm indicates that PTIO⁺· free radical ions have been scavenged, thereby enabling quantitative analysis of the PTIO scavenging capacity of the sample.