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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
生物体的脱氢酶(Plant dehydrogenase,PDHA)的活性在很大程度上反映了生物体的活性状态,能直接表示生物细胞对其基质降解能力的强弱。在该试剂盒中,受氢体2,3,5-氯化三苯基四氮唑(2,3,5-Triphenyl Tetrazolium Chloride,即 TTC)在细胞呼吸过程中接受氢以后,其还原产物三苯基甲替(Triphenyl Formazone,即 TFF)呈现红色,在波长 485 nm 处有最大吸收峰,采用分光光度法于 485 nm 测定其吸光值,即得植物脱氢酶活性。
The activity of dehydrogenase in organisms (Plant dehydrogenase, PDHA) largely reflects the metabolic state of the organism and directly indicates the capacity of biological cells to degrade substrates. In this assay kit, the hydrogen acceptor 2,3,5-Triphenyl Tetrazolium Chloride (TTC) accepts hydrogen during cellular respiration, and its reduction product, Triphenyl Formazone (TFF, a red-colored compound), exhibits a maximum absorption peak at a wavelength of 485 nm. The activity of plant dehydrogenase is determined by measuring the absorbance at 485 nm using a spectrophotometric method.