BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
原花青素（Oligomeric Proantho Cyanidins，OPC）是一类黄烷醇单体及其聚合体的多酚化合物，广泛存在于植物的各种器官中，具有极强的抗氧化性和清除自由基的作用，广泛的应用于医药，食品，化妆品，保健品行业。
测定原理：
在酸性条件下，植物原花青素A环上的间苯二酚和间苯三酚与香草醛发生缩合反应，产生有色化合物，在500nm处有特征吸收峰，测定500nm光吸收值可计算原花青素的含量。正式测定前务必取2-3个预期差异较大的样本做预测定。
注：本试剂盒仅限科研使用，不可用于临床诊断及其他用途。
试剂一：8%盐酸，自备（1.6mL 盐酸溶于 18.4mL 甲醇）4℃保存。 试剂二：粉剂0.125g×1瓶，4℃避光保存，临用前加12.5mL甲醇溶解。 测定工作液：临用前按照用量将试剂一和试剂二按照1:1混合。 空白工作液：临用前按照用量将试剂一和甲醇按照1:1混合。 |
自备仪器和试剂
OPC提取：
称取约0.1g样本，加入2ml提取液，匀浆后，60℃振荡提取2h， 10000g，25℃，离心10min，取上清待测。
测定操作表
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OPC计算公式：
标准曲线：y=0.0194x+0.0006，R²=0.999
OPC含量 (mg/g 鲜重) = (ΔA-0.0006)÷0.0194×反总÷(样÷样总×W)×10⁻³= 0.515×(ΔA-0.0006 ) ÷ W
V样总：加入提取液体积，2ml
V反总：反应总体积，1ml
V样：反应中样品体积，0.2ml
W：样品质量，g
注意事项：
1、配制好的试剂二应尽快使用，4℃保存时间不超过一个月。
2、吸光度变化应该控制在0.05~0.8之间。否则加大样品量或稀释样品，注意计算公式中参与计算的稀释倍数要相应改变。
3、最低检出限为10μg/g。
Proanthocyanidins (Oligomeric Proanthocyanidins, OPC) are a group of polyphenolic compounds composed of flavanol monomers and their polymers. They are widely distributed in various plant organs and exhibit strong antioxidant activity and free radical scavenging effects. Accordingly, they are extensively applied in the pharmaceutical, food, cosmetic and health product industries.
Determination Principle:
Under acidic conditions, resorcinol and phloroglucinol on the A-ring of plant proanthocyanidins undergo a condensation reaction with vanillin to form colored compounds, which have a characteristic absorption peak at 500 nm. The proanthocyanidin content can be calculated by measuring the absorbance at 500 nm. It is necessary to conduct a pre-experiment with 2–3 samples of expected significant differences before formal detection.
Note: This kit is only for scientific research purposes and shall not be used for clinical diagnosis or other applications.
Reagent Ⅰ: 8% hydrochloric acid, self-prepared (Dissolve 1.6 mL hydrochloric acid in 18.4 mL methanol). Store at 4 °C. Reagent Ⅱ: Powder, 0.125 g per bottle. Store at 4 °C away from light; dissolve with 12.5 mL methanol before use. Working Assay Solution: Mix Reagent Ⅰ and Reagent Ⅱ at a volume ratio of 1:1 according to required dosage before use. Blank Working Solution: Mix Reagent Ⅰ and methanol at a volume ratio of 1:1 according to required dosage before use. |
Reagents, consumables and Equipments not provided
⁕Spectrophotometer (measurable at 500 nm)
⁕Electronic balance, room-temperature centrifuge, visible spectrophotometer, 1 mL glass cuvettes
⁕Distilled water, hydrochloric acid, absolute ethanol and methanol
OPC Extraction:
Weigh approximately 0.1 g of sample, add 2 mL extraction buffer. After homogenization, perform shaking extraction at 60 °C for 2 h. Centrifuge at 10000 g and 25 °C for 10 min, then collect the supernatant for subsequent determination.
Assay Protocol Table
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OPC Calculation Formula:
Standard curve equation: y=0.0194x+0.0006，R²=0.999
OPC content (mg/g fresh weight) = (ΔA-0.0006) ÷0.0194×Vtotal reaction÷(Vsample÷Vtotal sample×W)×10⁻³= 0.515×(ΔA-0.0006 ) ÷ W
Vtotal sample: Volume of added extract, 2 mL
Vtotal reaction: Total reaction volume, 1 mL
Vsample: Sample volume in reaction system, 0.2 mL
W: Sample weight, g
Precautions:
1. The prepared Reagent 2 should be used promptly. The storage period at 4 ℃ shall not exceed one month.
2. The absorbance value should be controlled within the range of 0.05–0.8. If out of range, increase the sample dosage or dilute the sample correspondingly, and adjust the dilution factor in the calculation formula.
3. The limit of detection (LOD) is 10 μg/g.
| 项目号 | 物理外观 | 组件 | 50T | Storage |
| - | 液体 | 提取液：60%乙醇 (自备) | 50 mL | 2-8℃. |
| - | 液体 | 试剂一：8%盐酸 (自备) | 20 mL | 2-8℃. |
| P1521900A | 固体 | 试剂二：粉剂 | 1 EA | 2-8℃. Store in the dark. |
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0838765 |
分析证书 | P1521900 |
| ZJ26F0535631 |
分析证书 | P1521900 |
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