计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
磷酸戊糖途径中,6-磷酸葡萄糖脱氢酶(G6PDH)和 6-磷酸葡萄糖酸脱氢酶(6PGDH)依次催化NADPH 合成,与能量的平衡、生长速率和细胞活力等密切相关。此外,6PGDH 逆境生理中具有重要作用。该产品提供了一种简单、方便、快速的 6PGDH 活性检测方法,适用于检测动物组织、植物组织、细胞和细菌、血清(浆)等样本。其原理是 6PGDH 催化 6-磷酸葡萄糖酸和NADP⁺生成 NADPH,NADPH 在 340 nm 有特征吸收峰,而NADP⁺没有;通过测定 340 nm 吸光度增加速率,计算 6PGDH 活性。
In the pentose phosphate pathway, glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) sequentially catalyze the synthesis of NADPH, which is closely related to energy balance, growth rate, and cell viability. Furthermore, 6PGDH plays an important role in stress physiology. This product provides a simple, convenient, and rapid method for assaying 6PGDH activity, suitable for detecting samples such as animal tissues, plant tissues, cells and bacteria, and serum (plasma). Its principle is that 6PGDH catalyzes the reaction between 6-phosphogluconate and NADP⁺ to generate NADPH. NADPH has a characteristic absorption peak at 340 nm, while NADP⁺ does not. 6PGDH activity is calculated by measuring the rate of increase in absorbance at 340 nm.