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磷酸戊糖途径途径中6-磷酸葡萄糖脱氢酶（G6PDH）和6PGDH依次催化NADPH合成，与能量的平衡、生长速率和细胞活力等密切相关。此外，6PGDH逆境生理中具有重要作用。 测定原理：6PGDH催化6-磷酸葡萄糖酸和NADP+生成NADPH，NADPH在340 nm有特征吸收峰，而NADP+没有；通过测定340nm吸光度增加速率，计算6PGDH活性。
6-Phosphogluconate dehydrogenase (6PGDH) sequentially catalyzes NADPH synthesis together with glucose-6-phosphate dehydrogenase (G6PDH) in the pentose phosphate pathway, and is closely associated with energy balance, growth rate, and cell viability. In addition, 6PGDH plays an important role in stress physiology. Determination Principle: 6PGDH catalyzes the conversion of 6-phosphogluconate and NADP⁺ to produce NADPH. NADPH exhibits a characteristic absorption peak at 340 nm, while NADP⁺ does not. 6PGDH activity is calculated by measuring the rate of increase in absorbance at 340 nm.
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