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天然果胶类物质以原果胶、果胶(Pectin)、果胶酸的形态广泛存在于植物的果实、根、茎、叶中，是细胞壁的一种组成成分，它们伴随纤维素而存在，构成相邻细胞中间层粘结物，使植物组织细胞紧紧黏结在一起。原果胶是不溶于水的物质，但可在酸、碱、盐等化学试剂及酶的作用下，加水分解转变成水溶性果胶。果胶(Pectin)又称多聚半乳糖醛酸，是由D-半乳糖醛酸以α-1,4糖苷键连接形成的直链状聚合物，本质上是一种线形的多糖聚合物，含有数百至约1000个脱水半乳糖醛酸残基，其相应的平均相对分子质量为50000~150000。原果胶是一种非水溶性的物质，在未成熟的果实或植物组织中果胶物质大多与纤维素结合以原果胶的形式存在，原果胶能使果实或其他植物组织坚硬、变脆。 该试剂盒检测原理是果胶物质水解生成半乳糖醛酸，后者在硫酸溶液中与咔唑进行缩合反应形成紫红色的化合物，该化合物呈色强度与半乳糖醛酸浓度成正比，该化合物颜色在反应1~2h内呈色最深，当反应液颜色最深时在波长530nm处测定吸光度，通过与标准曲线比较，计算出样品中原果胶或可溶性果胶含量。该试剂盒主要用于定量检测植物组织或果实中原果胶含量，亦可用于定量检测植物组织或果实中可溶性果胶含量，进而计算出总果胶含量(为原果胶含量与可溶性果胶含量之和)。本产品仅用于科研领域，不宜用于临床诊断或其他用途。
Natural pectin substances exist widely in the fruits, roots, stems, and leaves of plants in the forms of protopectin, pectin, and pectic acid. They are components of the cell wall, accompanying cellulose, and constitute the intercellular adhesive layer between adjacent cells, firmly bonding plant tissue cells together. Protopectin is a water-insoluble substance, but under the action of chemical reagents such as acids, alkalis, or salts, as well as enzymes, it can undergo hydrolysis and transform into water-soluble pectin. Pectin, also known as polygalacturonic acid, is a linear polymer composed of D-galacturonic acid units linked by α-1,4 glycosidic bonds. It is essentially a linear polysaccharide polymer containing several hundred to about 1000 anhydrogalacturonic acid residues, with a corresponding average relative molecular mass ranging from 50,000 to 150,000. Protopectin is a water-insoluble substance. In immature fruits or plant tissues, pectin substances are mostly combined with cellulose and exist in the form of protopectin, which makes the fruits or other plant tissues hard and crisp. The detection principle of this kit is as follows: Pectin substances are hydrolyzed to generate galacturonic acid, which then undergoes a condensation reaction with carbazole in a sulfuric acid solution to form a purplish-red compound. The color intensity of this compound is proportional to the concentration of galacturonic acid. The color of the compound reaches its maximum depth within 1-2 hours of the reaction. When the reaction solution exhibits the deepest color, the absorbance is measured at a wavelength of 530 nm. By comparing with a standard curve, the content of protopectin or soluble pectin in the sample is calculated. This kit is primarily used for the quantitative determination of protopectin content in plant tissues or fruits, and can also be used for the quantitative determination of soluble pectin content in plant tissues or fruits, thereby allowing the calculation of total pectin content (which is the sum of protopectin content and soluble pectin content). This product is for research use only and is not intended for use in diagnostic procedures.
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