BioReagent,比色法,适用于分析 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
植物酚类物质具有清除自由基，抗氧化，抗衰老的作用，具有较高的营养价值和医疗保健作用而广泛应用于化妆品、食品、医药等领域。本试剂盒检测原理是在碱性条件下，酚类物质将钨钼酸还原，产生蓝色化合物，在760 nm处有特征吸收峰，测760 nm 处的吸光值，即可得样品总酚含量。可以检测植物组织、果汁和蜂蜜等液体样本。
组份表：
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实验器材：
烘箱、粉碎仪、40目筛、超声破碎仪、研钵、离心机、 可见分光光度计、1 mL玻璃比色皿。
标准品制备：
操作步骤：
1.总酚提取：
注：上清液在测试前需取一个正常组的样本上清和一个预期差异较大的样本上清进行预试以确定最佳取 样浓度(测定管OD-对照管OD≤0.9)。
2.测定操作表：
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计算公式：
植物总酚含量(μmol/g组织)=(( A 测定 - A 对照 )/(A 标准 - A 空白 )) × C 标准 × V 样总 × N ÷ W
C标准 : 标准品浓度，500 μmol/L；
V样总 : 上清液总体积，0.002 L；
N: 上清液稀释倍数；
W:样本质量,g。
注意事项：
1、试剂一对皮肤有一定的刺激性，操作时做好防护措施。
2、本实验可自行制作标准曲线计算，也可按照操作表操作后按计算公式计算，不影响结果。
3、样本根据客户自己要求烘干或用鲜样都可，结果单位用μg/g组织表示时应乘以相应植物总酚平均分子质 量。
4、室温25℃时，可直接在室内条件下反应。
5、反应时尽量避免强光照射。
6、本试剂盒仅用于科研。
附录 植物总酚标准曲线制备(选做)
实验前准备： 吸取标准品母液100 uL，加入900 uL 60%乙醇，混匀得到1 mg/mL的标品稀释液待用，将2000 μmol/L标准品用60%乙醇水溶液稀释成1000 μmol/L、500 μmol/L、250 μmol/L、125 μmol/L几个浓度。
操作步骤：
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Plant phenolic compounds possess antioxidant, free radical scavenging, and anti-aging properties, offering high nutritional value and health benefits, and are widely used in cosmetics, food, pharmaceuticals, and other fields. The detection principle of this kit is based on the reduction of tungstomolybdic acid by phenolic compounds under alkaline conditions, forming a blue compound with a characteristic absorption peak at 760 nm. By measuring the absorbance at 760 nm, the total phenolic content of the sample can be determined. This method is suitable for testing plant tissues, fruit juices, honey, and other liquid samples.
Components：
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Experimental equipment:
Oven, grinder, 40-mesh sieve, ultrasonic disruptor, mortar, centrifuge, visible spectrophotometer, 1 mL glass cuvette.
Standard preparation:
Operation steps:
1.Total phenol extraction:
2. Measurement Operation Table:
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Calculation formula:
Total phenol content of plants (μmol/g tissue) = ((Aₜₑₛₜ - A<sub>Control</sub>) / (Aₛₜₐₙdₐᵣd - Abₗₐₙₖ)) × Cₛₜₐₙdₐᵣd × Vₛₐₘₚₗₑ × N ÷ W
Cₛₜₐₙdₐᵣd : Concentration of standard substance, 500 μmol/L;
Vₛₐₘₚₗₑ: Total volume of the supernatant, 0.002 L;
N: Dilution factor of the supernatant;
W: Sample mass, g.
Notes:
1. The reagents have a certain irritant effect on the skin. Take appropriate protective measures during the operation.
2. In this experiment, you can either create the standard curve yourself for calculation, or follow the operation table and then calculate using the given formula. This will not affect the results.
3. The samples can be dried according to the customer's own requirements or using fresh samples. When the result is expressed in μg/g of tissue, it should be multiplied by the average molecular weight of total phenols of the corresponding plant.
4. At a room temperature of 25℃, the reaction can be carried out under indoor conditions directly.
5. Try to avoid strong light exposure during the reaction process.
6. This kit is for scientific research purposes only.
Appendix Preparation of Standard Curve for Total Phenols in Plants (Optional)
Preparation before the experiment: Dilute the 2000 μmol/L standard solution with a 60% ethanol aqueous solution to obtain several concentrations of 1000 μmol/L, 500 μmol/L, 250 μmol/L, and 125 μmol/L.
Operation steps:
Note: Alternatively, a 2000 μmol/L standard solution can be prepared, and then diluted step by step to the desired gradient concentration. The sample addition system and the determination operation system should be consistent with those in the table. |
The diagram is as follows
| P1521997 | 组件 | 物理外观 | 48T | 储存 | 单次测试使用量 |
| P1521997A | 试剂一 | 黄色液体 | 8 mL | 2-8℃. 避光 | 每次反应需使用250 uL |
| P1521997B | 试剂二 | 无色透明液体 | 15 mL | 2-8℃ | 每次反应需使用250 uL |
| P1521997C | 标准品 | 白色粉末 | 10 mg | 2-8℃ | 按照需求使用 |
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0838233 |
分析证书 | P1521997 |
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