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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
脯氨酸是植物体内适应逆境胁迫的一种重要的渗透调节物质。高等植物中脯氨酸代谢因其初始底物不同,分为谷氨酸(Glu)和鸟氨酸(Orn)两条合成途径。吡咯啉-5-羧酸合成酶(pyrroline-5-carboxylate synthetase, P5CS)是以谷氨酸为前体合成脯氨酸途径的关键酶,对植物适应逆境胁迫起关键作用。该产品可检测植物组织等生物样本。在该试剂盒中,P5CS 催化谷氨酸生成 P5C 过程中分解 ATP 生成 ADP 和无机磷,通过钼酸铵比色法测定单位时间内产生无机磷的量可确定 P5CS 活性。
Proline is an important osmotic regulator in plants for adapting to environmental stress. In higher plants, proline metabolism, due to its different initial substrates, is divided into two synthesis pathways: the glutamate (Glu) pathway and the ornithine (Orn) pathway. Pyrroline-5-carboxylate synthetase (P5CS) is a key enzyme in the proline synthesis pathway using glutamate as a precursor and plays a crucial role in plant adaptation to environmental stress. This product can be used to detect biological samples such as plant tissues. In this assay kit, P5CS catalyzes the formation of P5C from glutamate, during which ATP is broken down into ADP and inorganic phosphorus. The activity of P5CS can be determined by measuring the amount of inorganic phosphorus produced per unit time using the ammonium molybdate colorimetric method.