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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
脯氨酸是植物体内适应逆境胁迫的一种重要的渗透调节物质。高等植物中脯氨酸代谢因其初始底物不同，分为谷氨酸( Glu) 和鸟氨酸( Orn) 两条合成途径。 吡咯啉-5-羧酸合成酶（P5CS，Δ1-pyrroline-5-carboxylate synthetase）是以谷氨酸为前体合成脯氨酸途径的关键酶，对植物适应逆境胁迫起关键作用。 测定原理：吡咯啉-5-羧酸合成酶催化谷氨酸生成P5C过程中分解ATP生成ADP和无机磷，通过钼酸铵比色法测定单位时间内产生无机磷的量可确定P5CS活性。
Proline is an important osmotic regulator in plants under stress conditions. In higher plants, proline metabolism follows two synthetic pathways based on different initial substrates: the glutamate (Glu) pathway and the ornithine (Orn) pathway. Δ1-Pyrroline-5-carboxylate synthetase (P5CS) is a key enzyme in the proline synthesis pathway that uses glutamate as a precursor and plays a crucial role in plant adaptation to stress. Determination Principle: During the catalysis of glutamate to P5C, P5CS decomposes ATP into ADP and inorganic phosphate. The activity of P5CS is determined by measuring the amount of inorganic phosphate produced per unit time using the ammonium molybdate colorimetric method.
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