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植酸酶（phytase）是催化植酸及其盐类水解为肌醇与磷酸（盐）的一类酶的总称，属磷酸单酯水解酶，它能将食品和饲料中植酸及其盐转化为可供有机体利用的有效磷，降低粪便中的磷含量，减轻对环境的污染，改善营养成分的吸收和利用，因此具有极其广泛的研究和应用价值。 测定原理：植酸酶在一定温度和pH值条件下，水解底物植酸钠生成无机磷与肌醇衍生物，无机磷在酸性环境中与钼酸铵显色剂反应生成蓝色复合物，在700nm处有特征吸收峰，根据700nm处吸光值变化可计算得植酸酶活性。
Phytase is a general term for a class of enzymes that catalyze the hydrolysis of phytic acid and its salts into inositol and phosphoric acid (or phosphates). Belonging to the family of phosphomonoesterases, it can convert phytic acid and its salts in food and feed into available phosphorus for organisms, reduce phosphorus content in feces, mitigate environmental pollution, and improve the absorption and utilization of nutrients. Therefore, it holds extensive research and application value. Determination Principle: Under specific temperature and pH conditions, phytase hydrolyzes the substrate sodium phytate to generate inorganic phosphorus and inositol derivatives. In an acidic environment, the inorganic phosphorus reacts with an ammonium molybdate chromogenic agent to form a blue complex, which exhibits a characteristic absorption peak at 700 nm. Phytase activity can be calculated based on the change in absorbance at 700 nm.
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