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焦磷酸：果糖-6-磷酸-1-磷酸转移酶（PFP，EC2.7.1.90）是一种胞质酶，广泛存在于植物组织中，催化果糖-6-磷酸与果糖-1,6-二磷酸之间的可逆转化，在光合作用碳代谢中起重要作用。 测定原理：PFP催化6-磷酸果糖转化为1,6-二磷酸果糖，它在醛缩酶和磷酸丙糖异构酶的作用下转变为3-磷酸甘油醛，再由3-磷酸甘油醛脱氢酶和NADH催化生成3-磷酸甘油酸、NAD和磷酸，340nm处的吸光度变化反映了PFP的活性的高低。
Pyrophosphate: fructose-6-phosphate-1-phosphotransferase (PFP, EC 2.7.1.90) is a cytosolic enzyme widely present in plant tissues. It catalyzes the reversible conversion between fructose-6-phosphate and fructose-1,6-bisphosphate and plays an important role in carbon metabolism during photosynthesis. Determination Principle: PFP catalyzes the conversion of fructose-6-phosphate to fructose-1,6-bisphosphate, which is then converted to glyceraldehyde-3-phosphate via aldolase and triose phosphate isomerase. Glyceraldehyde-3-phosphate is further catalyzed by glyceraldehyde-3-phosphate dehydrogenase in the presence of NADH to produce 3-phosphoglycerate, NAD⁺, and inorganic phosphate. The change in absorbance at 340 nm reflects the activity of PFP.
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