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PTM 是 PichiaTrace Minerals 的缩写,意为毕赤酵母微量元素,PTM1 是 PichiaPink1、2、3、4 系列菌株常用的一种 发酵用微量元素配方,不在种子液或接菌时发酵初始培养基中使用,多在流加营养液时添加,现也用于其他毕赤酵母发酵过 程的流加试验中。毕赤酵母的发酵过程分为四个阶段:1, 种子液培养阶段;2, Glycerolbatchphase,接菌开始到初始培 养基中甘油被用完的阶段,这一阶段的主要目的是积累细胞,为后续诱导表达做准备;3, 甘油流加阶段,此阶段不诱导蛋 白表达,仅用于增加生物量;4, 甲醇流加诱导阶段,此阶段主要用来诱导蛋白;PTM1 主要用于后两个阶段。
产品组分与配方:
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使用方法:
PTM1 已经 0.22um 过滤除菌,可直接添加使用。PTM1 微量元素母液,主要为毕赤酵母发酵试验补充微量元素,不同毕赤酵母发酵方法不同,具体要参考所用 protocol,比如常用的 PichiaPink 1、2、3、4 酵母菌株,甘油流加阶段:启动 50% 甘油 (含12mL/LPTM1) 流加,速率为 5–15mL/(h・L 初始发酵体积),持续 4 小时以上,使湿细胞重增至 150–220g/L。
注意事项:
1、 PTM1 微量元素母液为 0.22um 过滤除菌,可直接使用;若发现有杂菌污染,停止使用。
2、 PTM1 微量元素母液因浓度过高,底部可能会有少量沉淀产生,不影响使用,加入培养基后会重新溶解。
PTM is the abbreviation for Pichia Trace Minerals, referring to trace elements specially formulated for Pichia pastoris. PTM1 is a trace element formula for fermentation widely applied to the PichiaPink 1, 2, 3 and 4 strain series.It is not added to seed culture medium or the initial fermentation medium at inoculation, and is mostly supplemented together with fed-batch nutrients. At present, it is also adopted in fed-batch trials for fermentation of other Pichia pastoris strains.The fermentation process of Pichia pastoris consists of four phases:1. Seed culture stage. 2.Glycerol batch phase: the period from inoculation until complete depletion of glycerol in the initial medium. This phase mainly aims to accumulate biomass and prepare for subsequent induced protein expression.3.Glycerol fed-batch phase: no protein induction is carried out in this stage, whose sole purpose is to boost biomass concentration.4. Methanol fed-batch induction phase: this phase is dedicated to inducing target protein expression.
Product Composition and Formula:
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Instructions for Use:
PTM1 is sterile-filtered through a 0.22 μm filter and can be added directly for use.PTM1 Trace Element Stock Solution is designed to supplement trace elements during Pichia pastoris fermentation experiments.Fermentation protocols differ for various Pichia pastoris strains, so please follow your specific protocol accordingly.Taking the commonly used PichiaPink 1, 2, 3 and 4 strains as an example:During the glycerol fed-batch phase, start feeding 50% glycerol solution supplemented with 12 mL/L PTM1 at a feeding rate of 5–15 mL/(h·L initial fermentation volume). Continue feeding for no less than 4 hours until the wet cell weight reaches 150–220 g/L.
Precautions:
1. This PTM1 Trace Element Stock Solution has undergone 0.22 μm sterile filtration and is ready for direct use. Discontinue use immediately if bacterial contamination is detected.
2. A small amount of precipitate may form at the bottom of the solution due to its high concentration. This does not affect product performance, and the precipitate will dissolve completely once mixed into the culture medium.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | P1524575 | |
| 分析证书 | P1524575 |
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