计算溶液所需的质量、体积或浓度。
原代细胞保护剂 (1000×)
有货
为什么选择此级别
BioReagent,用于细胞培养 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
原代细胞保护剂，包含保护剂A、B组分，两种组分配合的应用到原代细胞分离培养过程中，可以有效预防和治疗原代细胞受到多种耐药菌、支原体、衣原体及其他病原微生物的污染。原代细胞的培养是指直接从机体取下细胞、组织和器官后立即进行培养。在原代细胞分离过程极易携带实验机体内的病原微生物或受到环境中微生物的污染，如出现培养液变浑浊、pH降低、细胞胞内颗粒、细胞变圆、易脱落，细胞间隙出现小黑点且冲洗不掉，或细胞间隙的小黑点在跳动等细胞病理状态。
组分与储存：
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使用说明：
1. 推荐保护剂A、B与培养液的稀释比例为1:1000，分别加入到分瓶培养的细胞悬液。
例如：10mL培养基分别加入10μL的保护剂A 和保护剂B。
2. 原代细胞换液的时候按比例加入保护剂A、B，可长期使用，用以预防和处理原代细胞出现多种病原微生物的污染。
悬浮细胞：悬浮细胞培养在未达到饱和密度时，培养基中的营养成分不能维持细胞的营养需求时，只能采用半量换液，需按照加入的培养液量按比例加入保护剂A、B。
贴壁细胞：消化液处理组织来源的细胞需充分漂洗，尽量除去消化液，细胞接种时浓度要稍大一些，在完全培养液中按比例加入保护剂A、B。
注意事项：
1. 本品不得与除青链霉素双抗外其它抗生素混用。
2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
3. 本试剂仅供研究使用。
Protective Agent For Primary Cell consists of Component A and Component B. When the two components are used in combination during the isolation and culture of primary cells, they can effectively prevent and remedy contamination of primary cells by multiple drug‑resistant bacteria, mycoplasmas, chlamydiae and other pathogenic microorganisms.
Primary cell culture refers to the immediate culture of cells, tissues or organs excised directly from an organism. During primary‑cell isolation, samples are highly prone to carrying pathogenic microorganisms from the donor organism or becoming contaminated by environmental microbes. Typical pathological manifestations include turbid culture medium, decreased pH, intracellular granular inclusions in cells, cell rounding, increased cell detachment, persistent non‑washable tiny dark spots in intercellular spaces, or motile tiny dark spots among cells.
Contents & Storage:
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Instructions for Use:
1. The recommended dilution ratio of Protective Agent A and Protective Agent B to culture medium is 1:1000. Add them separately to the cell suspension for sub‑culturing into divided flasks.
Example: Add 10 μL of Protective Agent A and 10 μL of Protective Agent B respectively into 10 mL culture medium.
2. Add Protective Agent A and B proportionally during medium change for primary cells. It can be used long‑term to prevent and address contamination of primary cells caused by various pathogenic microorganisms.
Suspension cells: For suspension cell culture before reaching saturated density, when nutrients in the medium can no longer meet cellular nutritional requirements, half‑volume medium exchange should be performed only. Add Protective Agent A and B proportionally according to the volume of fresh medium added.
Adherent cells: Tissue‑derived cells treated with digestive solution shall be thoroughly rinsed to remove digestive solution as much as possible. Seed cells at a slightly higher seeding density, and add Protective Agent A and B proportionally into complete culture medium.
Precautions:
1. This product shall not be mixed with antibiotics other than penicillin‑streptomycin double‑antibiotic.
2. For your safety and health, please wear a lab coat and disposable gloves during operation.
3. This reagent is for research use only.
规格
别名
原代细胞抗菌保护剂 | 原代细胞抑菌剂 | 原代细胞培养保护剂
英文别名
Primary‑cell protective agent | Primary cell culture protective supplement
规格或纯度
BioReagent, 用于细胞培养, 1000×
稳定性与储存
Store at -20℃ long term (12 months).
英文名称
Protective Agent For Primary Cell (1000×)
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
产品线
生物产品
名称和识别符
分子类型
试剂盒
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
技术文档和文章
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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