脑脊液 (Cerebro-Spinal Fluid，CSF) 是存在于脑室、蛛网膜下腔和脊髓中央管内的无色透明液体，由脑室中的脉络丛产生，与血浆和淋巴液的性质相似，正常成年人的脑脊液约 100~150ml，弱碱性，不含红细胞，具有一定的化学成分和压力，对维持颅压的相对稳定有重要作用，当中枢神经系统受损时，脑脊液的检测成为重要的辅助诊断手段。潘氏试剂 (Pandy) 主要成分为苯酚，脑脊液中球蛋白与苯酚结合，可形成不溶性蛋白盐而下沉，产生白色浑浊或沉淀，该检测法又称潘氏 (Pandy) 实验，多用于人或动物脑脊液的蛋白定性检查。本试剂仅用于科研领域，不适用于临床诊断或其他用途。
操作步骤 (仅供参考):
1、取小试管，加入 2~3 ml Pandy Reagent。
2、滴加脑脊液 1~2 滴 (约 0.05~0.1ml)，混匀，黑色背景下立即观察。
结果判断 ：
| 结果 | 现象 | | 阴性 | 清晰透明，不显雾状 | | 极弱阳性 (±) | 微呈白雾状，在黑色背景下才可见。 | | 阳性 (+) | 灰白色云雾状 | | (2+) | 白色浑浊 | | (3+) | 白色浓絮状沉淀 | | (4+) | 白色凝块 |
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注意事项
1、样本收集后应立即送检，一般不应超过 1h。
2、正常时多为阴性或极弱阳性，有脑组织和脑脊髓疾患时呈阳性反应。
3、试剂开封后请尽快使用，以防影响后续实验效果。
Cerebrospinal Fluid (CSF) is a colorless and transparent fluid found in the cerebral ventricles, subarachnoid space and central canal of the spinal cord. It is secreted by the choroid plexus within the ventricles and bears similar properties to blood plasma and lymph. In healthy adults, the total volume of CSF is approximately 100~150 mL. It is weakly alkaline and free of red blood cells. Containing specific chemical components and pressure, CSF plays a vital role in maintaining stable intracranial pressure. When the central nervous system is damaged, CSF examination serves as a crucial auxiliary diagnostic method.Pandy’s Reagent is mainly composed of phenol. The globulins in cerebrospinal fluid bind with phenol to form insoluble protein salts that precipitate, resulting in white turbidity or sediment. This assay is also known as the Pandy test, which is widely applied for qualitative detection of protein in human or animal cerebrospinal fluid. This reagent is intended for research use only and shall not be used for clinical diagnosis or other purposes.
Operating Procedures (For Reference Only):
1. Take a small test tube and add 2~3 mL of Pandy’s Reagent.
2. Add 1~2 drops (approximately 0.05~0.1 mL) of cerebrospinal fluid, mix thoroughly, and observe immediately against a black background.
Result Interpretation:
| Result | Observation | | Negative | Clear and transparent, no opalescence | | Weakly positive (±) | Slight white opalescence, only visible against a black background | | Positive (+) | Grayish white opalescence | | (2+) | White turbidity | | (3+) | Thick white flocculent precipitate | | (4+) | White clot |
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Precautions:
1. The sample shall be sent for test immediately after collection, and the waiting time generally shall not exceed 1 hour.
2. The result is mostly negative or weakly positive under normal conditions; positive reactions will appear when lesions occur in brain tissue or cerebrospinal cord.
3. Please use up the reagent as soon as possible after opening to avoid adverse effects on subsequent experiments.
ZJ26F0938863
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