计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
植物样本常用染色法包括整体样本染色和切片染色两种,整体染色更便于将染色结果进行组织级的原位标记,但由于植物组织本身含有的叶绿素、花青素等色素均会影响染色结果的观察。可通过脱色处理来得到更清晰的染色结果。
本产品性质稳定,脱色效果良好,能够在一定程度上重复使用,适用于植物组织整体染色后的脱色素处理。本产品仅用于科研领域,不适用于临床诊断或其他用途。
操作步骤(仅供参考):
1、将植物组织染色后样本置于植物组织脱色液 70-80℃ 脱色处理,直至充分去除背景颜色。该试剂在较高温度下脱色效果较好,脱色期间若溶液颜色较深,可更换 1-2 次新的植物组织脱色液。
2、观察染色结果并拍照记录,若不能及时观察结果,可将脱色后样本用水充分清洗后浸于植物组织保存液中保存以便后续观察。如长期浸泡保存建议放置 2-8℃,并至少 2 个月更换一次保存液。
注意事项:
1、试剂开封后请尽快使用,以防影响后续实验效果。
2、为了您的安全和健康,请穿实验服并戴一次性手套操作。
Common staining methods for plant samples include whole-mount staining and section staining. Whole-mount staining facilitates in situ labeling of staining signals at the tissue level. However, intrinsic pigments such as chlorophyll and anthocyanins within plant tissues will interfere with the observation of staining outcomes. Decolorization treatment can deliver clearer staining results.
This product features stable properties and excellent decolorizing performance, and it can be reused to a certain extent. It is designed for depigmentation after whole-mount staining of plant tissues. This reagent is only intended for scientific research and shall not be applied to clinical diagnosis or other purposes.
Operating Procedures (For Reference Only):
1. Immerse stained plant tissue samples in Plant Tissue Decolorization Solution and incubate at 70–80℃ to thoroughly eliminate background pigmentation. Higher temperatures can optimize the decolorizing effect. Replace the decolorization solution with fresh reagent 1 to 2 times if the liquid becomes heavily discolored during treatment.
2. Observe the staining effect and take photographic records. If immediate observation is not available, fully rinse the decolorized samples with clean water and store them in Plant Tissue Preservation Solution for later examination. For long-term immersion preservation, store the samples at 2–8 ℃ and replace the preservation solution at least once every two months.
Precautions:
1. Use the reagent as soon as possible after opening to avoid compromising experimental results.
2. Wear a lab coat and disposable gloves during operation to ensure personal safety and health.