丽春红(Ponceau S) 也叫 3 - 羟基 - 4-[2 - 磺酸基 - 4-(4 - 磺酸苯基偶氮) 苯基偶氮]-2,7 - 萘二磺酸四钠盐，分子式为C₂₂H₁₂N₄Na₄O₁₃S₄，分子量是 760.5688，CAS 号 6226-79-5，是一种水溶性阴离子重氮染料，由重氮氨基偶氮苯二磺酸和萘酚二磺酸耦合到四钠盐上。丽春红带负电荷，可以与带正电荷的氨基酸残基结合，同时丽春红也可以与蛋白的非极性区相结合，从而形成红色的条带。丽春红对蛋白的染色是可逆的，染色后可以用蒸馏水，PBS 或其它适当溶液洗去。染色液可以用于 PVDF 膜 (Western blots)、硝酸纤维素膜 (NC) 和醋酸纤维素膜 (Cellulose Acetate Membrane) 上的蛋白检测，但不适用于尼龙膜。丽春红 (Ponceau S) 染漂洗液主要应用于丽春红 (Ponceau S) 染色液的漂洗，主要是由乙酸等组成。该产品仅适用于科研实验，不可做他用。
保存条件：
室温保存，一年有效。
操作步骤 (仅供参考)：
1、按实验具体要求操作。
注意事项：
1、为了您的安全和健康，请穿实验服并戴一次性手套操作。
2、本产品仅用于科学研究实验用途，严禁用于临床诊断、人体注射、食品添加、化妆品生产及其他非科研场景。
3、使用者需自行做好个人防护与实验安全管控，若因违规操作、未按防护要求使用本试剂造成人身伤害、仪器损坏、环境污染等后果，均由使用方自行承担。
Ponceau S, also known as Tetrasodium 3-hydroxy-4-[2-sulfo-4-(4-sulfophenylazo)phenylazo]-2,7-naphthalenedisulfonate, has a molecular formula of C₂₂H₁₂N₄Na₄O₁₃S₄, a molecular weight of 760.5688 and a CAS number of 6226-79-5. It is a water-soluble anionic diazo dye, formed by the coupling of diazoaminoazobenzene disulfonic acid and naphthol disulfonic acid to its tetrasodium salt form.Carrying negative charges, Ponceau S can bind to positively charged amino acid residues, and at the same time, it can also combine with the non-polar regions of proteins, thus forming red bands. The staining of proteins with Ponceau S is reversible; after staining, the dye can be washed off with distilled water, PBS or other appropriate solutions. The staining solution can be used for protein detection on PVDF membranes (Western blots), nitrocellulose membranes (NC) and cellulose acetate membranes, but it is not suitable for nylon membranes.Ponceau S Staining Washing Solution is mainly used for rinsing Ponceau S Staining Solution, and is mainly composed of acetic acid and other components. This product is only suitable for scientific research experiments and shall not be used for any other purposes.
Storage Conditions:
Store at room temperature, valid for one year.
Operating Procedures (For Reference Only):
1. Operate in accordance with the specific requirements of the experiment.
Precautions:
1. For your safety and health, please wear a lab coat and disposable gloves during operation.
2. This product is only for scientific research and experimental use. It is strictly prohibited to use it for clinical diagnosis, human injection, food addition, cosmetic production and other non-research scenarios.
3. Users shall be responsible for their own personal protection and laboratory safety management. Any personal injury, equipment damage, environmental pollution and other consequences caused by improper operation or failure to use the reagent in accordance with the protection requirements shall be borne by the user.
ZJ26F0838440
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