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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
青霉素-链霉素溶液-多粘菌素B溶液是专门用于细胞培养的三抗,经过滤除菌,可以直接添加到细胞培养液内。其中,青霉素的含量为10kU/ml,链霉素的含量为10mg/ml,多粘菌素B的含量为25ug/ml。在细胞培养液中推荐的青霉素的工作浓度为100U/ml,链霉素的工作浓度为0.1mg/ml,多粘菌素B的工作浓度为0.25ug/ml。本品按照100倍稀释使用,即10ml青霉素-链霉素-多粘菌素B溶液(100×)可以配制1L细胞培养液。本产品生产过程遵循ISO9001及ISO13485体系指导原则。 主要成分:青霉素,链霉素,多粘菌素B,氯化钠。 操作方法 将本品37°C水浴快速溶解,待融化后摇匀,分装或直接按(100X)比例添加到试剂中,用于细胞培养的抗生素溶液,可以直接添加到细胞培养液内直接使用。整个使用操作过程皆应在无菌条件下操作。分装后未用的试剂立即储存于-20℃以下,避免反复冻融。 注意事项 1.本产品经过滤除菌,使用时应注意无菌操作,避免污染; 2.为保持本产品的最佳使用效果,请勿反复冻融; 3.本产品仅用于科研或进一步研究使用,不用于诊断和治疗。
This product is a triple antibiotic solution specifically designed for cell culture. It has been sterilized by filtration and can be added directly to cell culture media. The concentrations are: penicillin 10 kU/mL, streptomycin 10 mg/mL, and polymyxin B 25 μg/mL. The recommended working concentrations in cell culture medium are: penicillin 100 U/mL, streptomycin 0.1 mg/mL, and polymyxin B 0.25 μg/mL. This product is intended for use at a 100‑fold dilution, i.e., 10 mL of penicillin‑streptomycin‑polymyxin B solution (100×) can be used to prepare 1 L of cell culture medium. The manufacturing process complies with ISO 9001 and ISO 13485 guidelines. Main Components Penicillin, streptomycin, polymyxin B, sodium chloride. Procedure Quickly thaw the product in a 37°C water bath. After thawing, mix thoroughly, then aliquot or directly add to the reagent at a (100×) ratio. As an antibiotic solution for cell culture, it can be added directly to the culture medium for immediate use. All handling operations should be performed under aseptic conditions. Unused aliquots should be stored immediately at ‑20°C or below, avoiding repeated freeze‑thaw cycles. Precautions 1. This product has been sterilized by filtration. Aseptic techniques must be observed during handling to prevent contamination. 2. To maintain optimal performance, avoid repeated freeze‑thaw cycles. 3. This product is intended for research or further study purposes only; it is not for diagnostic or therapeutic use.