植物根系对离子的吸收具有选择性，即使对环境中相同浓度的离子，吸收速率也不相同，如硫酸铵溶液，根系对铵离子的吸收比对硫酸根离子的吸收快，但对于硝酸钠溶液，根系对硝酸根的吸收快于钠离子的吸收。将阳离子吸收较多、阴离子吸收较少导致介质环境pH降低的盐，称为生理酸性盐；将阴离子吸收较多，阳离子吸收较少导致介质环境pH升高的盐，称为生理碱性盐。
植物生理酸性盐溶液 (阿拉丁货号: P1526083) 是指阳离子吸收较多、阴离子吸收较少导致介质环境pH降低的盐，植物生理碱性盐溶液 (阿拉丁货号：P1526084) 是指阴离子吸收较多，阳离子吸收较少导致介质环境pH升高的盐。植物生理对照盐溶液主要由磷酸二氢钾、硫酸镁、氯化钙等组成，pH约为5.8，其中磷酸二氢钾浓度为0.0125%、硫酸镁浓度为0.0125%，不含硫酸铵和硝酸钠。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
操作步骤 (仅供参考)
1、挑选大小一致、健壮的植株，用刀片去掉胚乳后作为实验材料。
2、以黑色塑料薄膜蒙住瓶口，用橡皮筋扎紧，在薄膜上用镊子钻多个小孔，将株苗的根从小孔伸入溶液中，常温培养。
3、三天后取出植株，用蒸馏水将溶液补足到标记高度后混匀并测定其pH值。
4、按下表测定、计算实验结果：
| 氮源 | 实验前pH | 实验后pH | ΔpH | | 植物生理酸性盐溶液 | (NH₄)₂SO₄ |
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| | 植物生理碱性盐溶液 | NaNO₃ |
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| | 植物生理对照液 | 对照 |
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计算：
通过实验组 ΔpH 与对照组 ΔpH 对比计算，可分析植株对溶液中阴、阳离子的相对吸收量。
注意事项
1、准确调节与测定溶液pH值。
2、选取植物株苗生长状况要一致，且不能伤及根系。
3、试剂开封后请尽快使用，以防影响后续实验效果。
4、为了您的安全和健康，请穿实验服并戴一次性手套操作。
Plants exhibit selectivity in ion uptake by roots. Even for ions at the same concentration in the environment, their absorption rates vary. For instance, in ammonium sulfate solution, plant roots absorb ammonium ions faster than sulfate ions; while in sodium nitrate solution, roots take up nitrate ions more rapidly than sodium ions.
Salts whose cations are absorbed more than anions, resulting in a drop in the pH of the medium, are defined as physiologically‑acidic salts. Salts whose anions are absorbed more than cations, leading to an increase in the pH of the medium, are defined as physiologically‑alkaline salts.
Plant Physiologically‑Acidic Salt Solution (Aladdin Cat.No.: P1526083) refers to salts where cations are absorbed in greater quantities than anions, which causes the pH of the medium to decrease.Plant Physiologically‑Alkaline Salt Solution (Aladdin Cat.No.: P1526084) refers to salts where anions are absorbed in greater quantities than cations, which causes the pH of the medium to increase.The plant physiological control salt solution is mainly composed of potassium dihydrogen phosphate, magnesium sulfate, calcium chloride, etc., with a pH of approximately 5.8. The mass concentration of potassium dihydrogen phosphate is 0.0125 %, and magnesium sulfate is 0.0125 %. It does not contain ammonium sulfate or sodium nitrate. This reagent is for scientific‑research use only and is not intended for clinical diagnosis or other applications.
Operating Procedures (For Reference Only)
1. Select robust plant seedlings of uniform size. Remove the endosperm with a razor blade to obtain experimental materials.
2. Cover the bottle mouth with black plastic film and fasten it with rubber bands. Punch several small holes in the film using tweezers. Insert the seedling roots through the holes into the solution, and incubate at ambient temperature.
3. After three days, take out the seedlings. Top up the solution with distilled water to the marked level, mix thoroughly, and measure its pH value.
4. Measure and calculate the experimental results according to the table below:
| Nitrogen Source | pH Before Experiment | pH After Experiment | ΔpH | | Plant physiologically‑acidic salt solution | (NH₄)₂SO₄ |
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| | Plant physiologically‑alkaline salt solution | NaNO₃ |
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| | Plant physiological control solution | Control |
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Calculation:
The relative uptake of anions and cations in solution by plant seedlings can be analyzed by comparing the ΔpH of the experimental group with that of the control group.
Precautions
1. Adjust and measure the pH value of the solution accurately.
2. Select plant seedlings with consistent growth status and avoid damaging their root systems.
3. Use the reagent as soon as possible after opening to prevent adverse effects on subsequent experimental results.
4. For your safety and health, wear a laboratory coat and disposable gloves during operation.
ZJ26F1040108
危险品化学品经营许可证（带存储）