Phospholipase C (PLC) from Bacillus cereus is useful for enzymatic determination of lecithin when coupled with alkaline phosphatase and choline oxidase.
Preparation and Specification
Appearance: White to brownish amorphous powder, lyophilized
Specific activity: ≥30 U/mg enzyme powder
Properties
Molecular weight: 20 kDa (Sephadex G–100)
Isoelectric point: pH 7.0
Michaelis constants: Phosphatidylcholine 2.0 × 10⁻² M
Optimum pH: 7.0–9.0
pH stability: 5.0–10.0 (in 0.1% BSA)
Thermal stability: Stable at 55°C and below (pH 7.5, 10 min)
Storage stability: At least one year at -20°C
Activator: Ca²⁺, Sodium deoxycholate
Inhibitor: Tris–HCl buffer
规格
产品名称
磷脂酶C (PLC) 来源于蜡样芽胞杆菌
规格或纯度
EnzymoPure™, 重组, ≥30 U/mg enzyme powder
CAS编号和信息
9001-86-9
酶学委员会编号
EC 3.1.4.3
分子类型
酶
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
浓度
≥30 U/mg enzyme powder
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
单位定义
One unit is defined as the amount of enzyme which liberates 1 μmole of phosphoryl choline per minute at 37℃ under the conditions specified in the assay procedure.