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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
丙酮酸脱氢酶（PDH，EC 1.2.4.1）广泛存在于动物、植物、微生物及培养细胞中，是丙酮酸脱氢酶复合体（PDHC）催化丙酮酸氧化脱羧的限速酶，在连接糖酵解与三羧酸循环中发挥关键作用。本试剂盒采用MTT比色法测定PDH活性：PDH催化底物丙酮酸钠脱羧生成羟乙基-TPP，羟乙基-TPP进一步将电子传递至噻唑蓝（MTT），在电子中介体吩嗪硫酸甲酯（PMS）存在下，MTT被还原生成蓝色甲臜（Formazan）产物，该产物在566 nm处具有特征吸收峰。通过动力学模式连续监测单位时间内OD₅₆₆的初始线性增幅（ΔOD₅₆₆/min），即可精准计算样品中PDH的酶活力大小。
Pyruvate dehydrogenase (PDH, EC 1.2.4.1) is widely distributed in animals, plants, microorganisms, and cultured cells. It is the rate-limiting enzyme of the pyruvate dehydrogenase complex (PDHC), catalyzing the oxidative decarboxylation of pyruvate, and plays a critical role in linking glycolysis to the tricarboxylic acid (TCA) cycle. This kit employs an MTT colorimetric method for the determination of PDH activity: PDH catalyzes the decarboxylation of the substrate sodium pyruvate to generate hydroxyethyl-TPP, which subsequently transfers electrons to thiazolyl blue tetrazolium bromide (MTT). In the presence of the electron mediator phenazine methosulfate (PMS), MTT is reduced to yield a blue formazan product, which exhibits a characteristic absorbance peak at 566 nm. By continuously monitoring the initial linear increase in OD₅₆₆ per unit time (ΔOD₅₆₆/min) in kinetic mode, the PDH enzyme activity in the sample can be precisely calculated.
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