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果胶甲酯酶（PE）属果胶酶系，又称果胶酯酶或果胶氧化酶。该酶催化果胶长链上甲氧酯的水解，生成小分子果胶酸和甲醇，从而增加果胶在水中的溶解度。PE广泛存在于高等植物以及可降解细胞壁的细菌和真菌中，在植物体内起内源调控细胞壁及胞间果胶含量的作用。 果胶甲酯酶催化水解果胶分子时释放H⁺，导致反应体系pH下降。通过滴加碱液维持体系pH恒定为7.8（以酚酞为指示剂，保持粉红色），根据所消耗的NaOH量即可反映果胶甲酯酶的活性。
Pectin methylesterase (PE), also known as pectinesterase or pectin oxidase, is a member of the pectinase enzyme family. It catalyzes the hydrolysis of methoxy ester bonds in the pectin long chain, generating low-molecular-weight pectic acid and methanol, thereby increasing the solubility of pectin in water. PE is widely distributed in higher plants, as well as in bacteria and fungi capable of degrading cell walls. In plants, it plays a role in the endogenous regulation of cell wall and intercellular pectin content. The hydrolysis of pectin molecules catalyzed by PE releases H⁺, resulting in a decrease in the pH of the reaction system. By titrating with alkali to maintain the system pH constant at 7.8 (using phenolphthalein as the indicator to retain a pink color), the amount of NaOH consumed reflects the activity of pectin methylesterase.
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