BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
磷酸葡萄糖变位酶（PGM）在碳水化合物代谢中发挥关键作用，广泛存在于所有生物体中。PGM催化葡萄糖-1-磷酸（G1P）与葡萄糖-6-磷酸（G6P）之间的可逆转化。糖原分解时，PGM将G1P转化为G6P，进入糖酵解途径产生ATP，或经戊糖磷酸途径生成核糖和NADPH；当细胞需要合成糖原时，PGM则将G6P转化为G1P。PGM缺乏可导致葡萄糖储存相关疾病。 本试剂盒提供一种简单、灵敏、快速的测定方法：PGM催化G1P异构化为G6P，G6P在葡萄糖-6-磷酸脱氢酶作用下氧化生成NADPH，NADPH与特异性显色探针反应生成有色产物，通过测定450 nm处该产物的生成速率，即可计算PGM活性。
Phosphoglucomutase (PGM) plays a key role in carbohydrate metabolism and is ubiquitously present in all living organisms. PGM catalyzes the reversible interconversion of glucose‑1‑phosphate (G1P) and glucose‑6‑phosphate (G6P). During glycogen breakdown, PGM converts G1P to G6P, which then enters glycolysis to produce ATP, or enters the pentose phosphate pathway to generate ribose and NADPH. When cells require glycogen synthesis, PGM converts G6P to G1P. PGM deficiency can lead to disorders related to glucose storage. This kit provides a simple, sensitive, and rapid method for PGM determination. PGM catalyzes the isomerization of G1P to G6P. G6P is then oxidized by glucose‑6‑phosphate dehydrogenase (G6PDH) to generate NADPH, which reacts with a specific chromogenic probe to form a colored product. By measuring the rate of formation of this product at 450 nm, the PGM activity can be calculated.
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