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磷脂酶 D（PLD，EC 3.1.4.4），即磷脂酰胆碱水解酶，是催化磷酸二酯键水解和碱基交换反应的一类酶的总称，广泛存在于高等动植物及细菌等多种生物体中，参与细胞脂质代谢、信号传导、生物膜形成及抗逆境胁迫等生理过程。 本试剂盒的测定原理为：PLD催化水解磷脂酰胆碱末端的磷脂酰二酯键，生成磷脂酸和胆碱；胆碱在胆碱氧化酶催化下生成甜菜碱和过氧化氢（H₂O₂）；H₂O₂在过氧化物酶（POD）催化下，将4-氨基安替比林和重蒸酚氧化生成粉红色醌亚胺化合物，该产物在500 nm处具有特征吸收峰。通过测定500 nm处吸光度的变化速率，即可计算PLD的酶活性。
Phospholipase D (PLD, EC 3.1.4.4), also known as phosphatidylcholine hydrolase, is a general term for a class of enzymes that catalyze the hydrolysis of phosphodiester bonds and base‑exchange reactions. It is widely distributed in higher plants, animals, bacteria, and other organisms, and participates in various physiological processes including cellular lipid metabolism, signal transduction, biofilm formation, and stress resistance. The assay principle of this kit is as follows: PLD catalyzes the hydrolysis of the terminal phosphodiester bond of phosphatidylcholine, generating phosphatidic acid and choline. Choline is then oxidized by choline oxidase to produce betaine and hydrogen peroxide (H₂O₂). In the presence of peroxidase (POD), H₂O₂ oxidizes 4‑aminoantipyrine and phenol to generate a pink quinone imine compound, which exhibits a characteristic absorption peak at 500 nm. By measuring the rate of change in absorbance at 500 nm, the PLD enzyme activity can be calculated.
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