BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
普鲁兰酶（Pullulanase）是一种广泛存在于微生物、动植物体内的水解酶，能专一性裂解淀粉、糖原及其衍生物分支点中的 α-1,6-葡萄糖苷键。该酶早期用于淀粉结构的基础理论研究，20世纪70年代起其应用逐步扩展至淀粉糖浆、啤酒酿造、酒精生产等淀粉深加工领域，已实现从实验室研究到工业化生产的跨越。 本试剂盒采用 DNS 比色法测定普鲁兰酶活力。其原理为：普鲁兰酶催化底物普鲁兰水解生成还原糖；还原糖与 3,5-二硝基水杨酸（DNS）在沸水浴条件下反应，生成棕红色的氨基化合物，该产物在 540 nm 处具有特征吸收峰。在既定线性范围内，540 nm 处吸光度的增幅与还原糖生成量呈正比，通过连续监测吸光度的增加速率，即可计算样品中普鲁兰酶的活力。
Pullulanase is a hydrolytic enzyme widely distributed in microorganisms, animals, and plants. It specifically cleaves the α‑1,6‑glycosidic bonds at the branch points of starch, glycogen, and their derivatives. This enzyme was initially used in fundamental research on starch structure. Since the 1970s, its applications have gradually expanded to starch deep‑processing fields such as starch syrup production, beer brewing, and alcohol production, marking a transition from laboratory research to industrial‑scale manufacturing. This kit employs the DNS colorimetric method for the determination of pullulanase activity. The principle is as follows: Pullulanase catalyzes the hydrolysis of the substrate pullulan to generate reducing sugars. The reducing sugars react with 3,5‑dinitrosalicylic acid (DNS) under boiling water bath conditions to form a brown‑red amino compound, which exhibits a characteristic absorption peak at 540 nm. Within the established linear range, the increase in absorbance at 540 nm is directly proportional to the amount of reducing sugars generated. By continuously monitoring the rate of increase in absorbance, the pullulanase activity in the sample can be calculated.
危险品化学品经营许可证（带存储）